Bone and vascular signals during the repair process after bone and bone marrow injury

血管内皮生长因子受体Flt-1酪氨酸激酶结构域的缺失干扰了小鼠成骨细胞的分化，延缓了骨和骨髓损伤后的修复过程。成骨细胞与内皮细胞共培养时碱性磷酸酶产量增加。这种增加被PECAM-1(血小板内皮细胞黏附分子-1)抗体剂量依赖性地抑制。这些结果表明，包括血管内皮生长因子和PECAM-1在内的血管因子在骨和骨髓损伤后的修复过程中对成骨细胞的发育起重要作用。

The dis ruption of tyrosine kinase domain of flt-1, one of VEGF (vascular endothelial growth factor) receptors, disturbed osteoblast differentia tion and delayed the repair process after bone and bone marrow injury in mice. The production of alkaline phosphatase increased in osteoblasts co-cultured with endothelial cells. This increase was dose-dependently inhibited by PECAM-1 (platelet endothelial cell adhesion molecule-1) antibody. These findings demonstrated that vascular factors including VEGF and PECAM-1 significantly contributed to osteoblast development in the repairprocess afterbone and bone marrow injury.