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Role of the bombesin-like peptide system in the network regulating energy metabolism

铃蟾肽样肽系统在调节能量代谢网络中的作用

基本信息

批准号：
18500237
负责人：
OHKI Hiroko
金额：
$ 2.63万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500237/
关键词：
fatty acids feeding regulation obesity bombesin-like-peptides BRS-3 Knock-out mouse

项目摘要

Fatty acids have an important role to convey energy state of the whole body to the brain. We studied whether the bombesin-like peptide system is involved in this regulation, by using BRS-3 (bombesin-like peptide receptor 3) knock-out mice.Knock-out mice at 30-weeks-old were obese, and fatty acids (palmityl acid, oleic acid, etc) content in the blood was elevated despite of its decrease in the brain. In the knock-out mice, feeding-suppressing effect of oleic acid injected intracerebroventricularly was decreased. On the other hand, octanoic acid did not affect the feeding in both wild and knock-out mice. Then, we used cFos expression as the neural activation marker. Administration of oleic acid into the wild type mice induced cFos expression in the hypothalamus, but in the knock-out mice this effect was not observed. Using in situ hybridization, it is shown that the BRS-3-positive cells in the arcuate nucleus of the hypothalamus did not express at detectable level feeding-promoting peptide such as NPY and AgRP, and feeding-suppressing peptide such as CART and POMC. When the isolated cells from the arcuate nucleus of the wild or knock-out mice were assayed by the calcium imaging technique, they responded to the fatty acids. The response of the BRS-3-expressing cells should be isolated and analyzed in this experiment in the future.From these results, we concluded that the BRS-3 expressing cells in the arcuate nucleus were involved in the regulation of the feeding by the fatty acids. These results demonstrated one aspect of mechanism of fatty acids action on the feeding, and are important because they may be associated with the incidence of the metabolic syndrome.

脂肪酸在将整个身体的能量状态传递给大脑方面起着重要的作用。我们通过敲除BRS-3（炸弹素样肽受体3）小鼠来研究炸弹素样肽系统是否参与了这种调节。30周大的敲除小鼠肥胖，血液中的脂肪酸（棕榈酰酸、油酸等）含量升高，尽管其在大脑中的含量降低。在敲除小鼠中，脑室内注射油酸抑制摄食的作用减弱。另一方面，辛酸对野生小鼠和敲除小鼠的摄食没有影响。然后，我们使用cFos表达作为神经激活标记。油酸注入野生型小鼠可诱导cFos在下丘脑的表达，但在敲除小鼠中未观察到这种作用。原位杂交结果显示，下丘脑弓形核brs -3阳性细胞不表达NPY、AgRP等促食肽，也不表达CART、POMC等促食肽。当用钙成像技术检测野生或基因敲除小鼠弓形核分离的细胞时，它们对脂肪酸有反应。brs -3表达细胞的反应需要在以后的实验中进行分离和分析。由此我们推断，弓形核中表达BRS-3的细胞参与了脂肪酸摄食的调控。这些结果证明了脂肪酸作用于摄食的一个方面的机制，因为它们可能与代谢综合征的发生有关。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior

端脑背侧大脑皮层中胆囊收缩素神经元的激活对于获得雏鸡印记行为是必不可少的

DOI：
发表时间：
2007
期刊：
J Neurochem 102
影响因子：
0
作者：
Maekawa;F.;Nakamori;T.;Uchimura,M.;Fujiwara;K.;Yada;T.;Tsukahara,S.;Kanamatsu;T.;Tanaka;K.;Ohki-Hamazaki;H
通讯作者：
H

Disruption of neuromedin B receptor gene results in dysregulation of the pituitary-thyroid axis.

DOI：
10.1677/jme.1.01892
发表时间：
2006-02
期刊：
Journal of molecular endocrinology
影响因子：
3.5
作者：
K. Oliveira;T. Ortiga-Carvalho;Adriana Cabanelas;M. A. L. Veiga;K. Aoki;Hiroko Ohki-Hamazaki;K. Wada;E. Wada;C. Pazos-Moura
通讯作者：
K. Oliveira;T. Ortiga-Carvalho;Adriana Cabanelas;M. A. L. Veiga;K. Aoki;Hiroko Ohki-Hamazaki;K. Wada;E. Wada;C. Pazos-Moura

Overexpression of gastrin-releasing peptide receptor induced layer disorganization in brain.

胃泌素释放肽受体的过度表达诱导脑层紊乱。