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The regeneration for spinal cord injuries by using pituitary folliculo-stellate cell grafts.

使用垂体滤泡星状细胞移植物再生脊髓损伤。

基本信息

批准号：
18591600
负责人：
SAKUMA Eisuke
金额：
$ 2.49万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The anterior lobe of a pituitary gland is comprised of hormone-producing granular cells and agranular cells. Agranular cells, characterized by their star-like morphological features and specific ability of forming follicles found within the adenohypophysis of many mammalian species, have been named folliculo-stellate cells. Folliculo-stellate cells extend slender cytoplasmic processes among the hormone-producing granular cells to form a cell network throughout the whole anterior pituitary gland and are also known to be coupled by gap junctions in normal mature rats. The folliculo-stellate cells have been thought to be tissue specific stem cells. The aim of this study was to examine the effect of transplantation of these cells over the regeneration within injured spinal cords of rats, by using the electron voltage investigations and histochemistry. We used the operation microscope (OPMI VISU 210, Carl Zeiss S88), coutary pen (Gemini Cautery Kit), light hardening resin (Uniflow; GC Co.), operation silicon plates (Dow Corning Co.,Midland, MI, USA), osmotic pump system (ALZET) and research microscope (Nikon IX-70). We achieved some methods for these transplantation regeneration methods and presented some academic papaers (Otsuka & Sakuma, et. al., 2006; Shirasawa & Sakuma, et. al., 2007).

脑下垂体的前叶是由产生分泌物的颗粒细胞和无颗粒细胞组成。无颗粒细胞是哺乳动物腺垂体中的一种细胞，具有星状的形态特征和形成滤泡的特殊能力，被称为滤泡星状细胞。滤泡-星状细胞在产生腺泡的颗粒细胞之间延伸细长的胞质突起，形成遍布整个垂体前叶的细胞网络，并且已知在正常成熟大鼠中通过间隙连接偶联。滤泡星状细胞被认为是组织特异性干细胞。本研究的目的是通过电子电压研究和组织化学方法，观察这些细胞移植对大鼠脊髓损伤后再生的影响。我们使用了手术显微镜（OPMI VISU 210，Carl Zeiss S88），coutary笔（Gemini烧灼套件），光硬化树脂（Uniflow; GC Co.），操作硅板（Dow Corning Co.，米德兰，MI，USA），渗透泵系统（ALZET）和研究显微镜（Nikon IX-70）。我们对这些移植再生方法进行了一些研究，并发表了一些学术论文（Otsuka & Sakuma，et.例如，2006; Shirasawa & Sakuma，et.例如，2007年）。