The regeneration for spinal cord injuries by using pituitary folliculo-stellate cell grafts.

脑下垂体的前叶由产生激素的颗粒细胞和无颗粒细胞组成。无粒细胞以其星形的形态特征和在许多哺乳动物的腺垂体中发现的特有的形成卵泡的能力为特征，被称为毛囊星状细胞。卵泡星状细胞在分泌激素的颗粒细胞之间延伸细长的细胞质突起，形成一个贯穿整个脑下垂体的细胞网络，在正常成熟的大鼠中也被认为是通过缝隙连接连接的。毛囊星状细胞一直被认为是组织特异性干细胞。本研究的目的是通过电压检测和组织化学的方法，观察这些细胞移植对大鼠损伤脊髓内再生的影响。我们使用了手术显微镜(OPMI VISU 210，Carl Zeiss S88)、铜笔(Gemini烧灼试剂盒)、光硬化树脂(Uniflow；GC Co.)、手术硅板(Dow Corning Co.，Midland，MI，USA)、渗透泵系统(ALZET)和研究显微镜(Nikon IX-70)。我们实现了这些移植再生方法的一些方法，并提出了一些学术上的木瓜(Otsuka&Sakuma，et.等人，2006年；Shirasawa&Sakuma等人。等人，2007年)。

The anterior lobe of a pituitary gland is comprised of hormone-producing granular cells and agranular cells. Agranular cells, characterized by their star-like morphological features and specific ability of forming follicles found within the adenohypophysis of many mammalian species, have been named folliculo-stellate cells. Folliculo-stellate cells extend slender cytoplasmic processes among the hormone-producing granular cells to form a cell network throughout the whole anterior pituitary gland and are also known to be coupled by gap junctions in normal mature rats. The folliculo-stellate cells have been thought to be tissue specific stem cells. The aim of this study was to examine the effect of transplantation of these cells over the regeneration within injured spinal cords of rats, by using the electron voltage investigations and histochemistry. We used the operation microscope (OPMI VISU 210, Carl Zeiss S88), coutary pen (Gemini Cautery Kit), light hardening resin (Uniflow; GC Co.), operation silicon plates (Dow Corning Co.,Midland, MI, USA), osmotic pump system (ALZET) and research microscope (Nikon IX-70). We achieved some methods for these transplantation regeneration methods and presented some academic papaers (Otsuka & Sakuma, et. al., 2006; Shirasawa & Sakuma, et. al., 2007).