The regeneration for spinal cord injuries by using pituitary folliculo-stellate cell grafts.

脑垂体前叶由产生激素的颗粒细胞和颗粒细胞组成。在许多哺乳动物的腺垂体中发现的具有星形形态特征和形成卵泡的特殊能力的颗粒细胞被命名为卵泡星状细胞。卵泡星状细胞在产生激素的颗粒细胞之间延伸细长的细胞质突起，形成贯穿整个垂体前叶的细胞网络，并且已知在正常成熟大鼠中通过间隙连接偶联。卵泡星状细胞被认为是组织特异性干细胞。本研究采用电子电压法和组织化学方法，观察这些细胞移植对损伤大鼠脊髓再生的影响。我们使用了操作显微镜（OPMI VISU 210, Carl Zeiss S88），笔（Gemini Cautery Kit），光硬化树脂（Uniflow; GC Co.），操作硅板（Dow Corning Co.,Midland， MI， USA），渗透泵系统（ALZET）和研究显微镜（Nikon IX-70）。我们实现了这些移植再生方法的一些方法并发表了一些学术论文（Otsuka & Sakuma, et. al., 2006; Shirasawa & Sakuma, et. al., 2007）。

The anterior lobe of a pituitary gland is comprised of hormone-producing granular cells and agranular cells. Agranular cells, characterized by their star-like morphological features and specific ability of forming follicles found within the adenohypophysis of many mammalian species, have been named folliculo-stellate cells. Folliculo-stellate cells extend slender cytoplasmic processes among the hormone-producing granular cells to form a cell network throughout the whole anterior pituitary gland and are also known to be coupled by gap junctions in normal mature rats. The folliculo-stellate cells have been thought to be tissue specific stem cells. The aim of this study was to examine the effect of transplantation of these cells over the regeneration within injured spinal cords of rats, by using the electron voltage investigations and histochemistry. We used the operation microscope (OPMI VISU 210, Carl Zeiss S88), coutary pen (Gemini Cautery Kit), light hardening resin (Uniflow; GC Co.), operation silicon plates (Dow Corning Co.,Midland, MI, USA), osmotic pump system (ALZET) and research microscope (Nikon IX-70). We achieved some methods for these transplantation regeneration methods and presented some academic papaers (Otsuka & Sakuma, et. al., 2006; Shirasawa & Sakuma, et. al., 2007).