魚類の浸透圧調節における即効性及び遅効性ホルモンの作用協関係

快效和慢效激素在调节鱼类渗透压方面的合作

• 批准号: 07J08270
• 负责人: ベントゥラ A (2007, 2009)
• 金额: $ 1.79万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07J08270/
• 关键词: 魚類; コルチゾル; 浸透圧; アンギオテンシン; ナトリウムペプチド; アドレノメデュリン; ACTH; 間腎腺; eel; Cortisol; Omsoregulation; Natriuretic peptide; 浸透圧調節

ウナギは淡水から海水まで幅広い塩分環境に適応する広塩性魚であり、浸透圧調節の研究において優れたモデルである。広塩性魚における浸透圧調節は複雑なメカニズムで、様々なホルモンにより制御されている。これらのホルモンは速効性と遅効性ホルモンに分けられている。遅効性ホルモンであるコルチゾルは様々な役割を果たしており、真骨魚類における浸透圧調節において最も重要なホルモンの一つであると考えられている。そこで、本研究で即効性ルモンであるナトリウム利尿ペプチドファミリー(NP)によるコルチゾルの分泌調節を調べた。近年、NPファミリーは7種類に多様化したと明らかになり、ウナギで6種類が同定されている。海水と淡水ウナギの間腎腺の組織培養液中に6つのNPをそれぞれ添加した結果、陽性対照として使用したACTHでは明らかなコルチゾル産生が認められたが、NPではコルチゾルの産生が促進されなかった。in vivoではANPとCNP1はそれぞれ海水ウナギと淡水ウナギでコルチゾルの分泌を促進するため、NPが他のホルモンを介して、間接的にコルチゾルの分泌を促進していると考えた。そこでNPとACTHを同時に添加したところ、海水の間腎腺において、ANPとVNPはACTHのコルチゾル産生能を増強して、淡水ウナギの間腎腺ではCNP-1とCNP-4がACTHの効果を増強することが明らかになった。更にANGとAMについても検討を行った。ANGは4種類(ANGI、ANGII、ANGIIIとANGIV)が血中に存在し、さらにウナギとシロサケは、N末端がアスパラギンのN-ANGIIだけではなく、血中でアミノ基転移酸素によリアスパラギン酸に変換したD-ANGも持っている。それぞれANGを添加した結果、淡水と海水でD-ANGIIのコルチゾル分泌促進はN-ANGIIと比較して3倍高かった。ANG-IIIはN-ANGIIと同程度の効果であった。AM1、AM2、AM5はコルチゾル分泌を促進しなかった。本研究の結果から、ウナギのNPによるコルチゾル分泌調節には、ACTH存在することが不可欠だと明らかになった。そのため、現在ACTH測定ELISA法を確立し、下垂体におけるNPファミリーによるACTHの分泌調節を調べている。

The area of fresh water and sea water is divided into different environmental conditions, such as environmental protection, environmental protection and environmental protection. Please make sure that you can make a copy of the information and make sure that you are not in control. I don't know what to do. I don't know what to do. This is the most important thing in the world. This is the most important thing. The purpose of this study is to determine the secretion of diuretics, diuretics, diuresis, diuresis, di In recent years, there have been six types of information systems, such as NPF7, which are the same as those of the same standard. In the tissue culture solution of fresh water and fresh water, the results of NP were added to the tissue culture solution, and the results were added to the tissue culture solution of fresh water and fresh water. The results were added to the tissue culture solution of fresh water, fresh water and fresh water, and the results were added to the tissue culture solution of fresh water and fresh water. The results of the tissue culture solution were added to the tissue culture solution of fresh water, fresh water and fresh water, and the results were added to the tissue culture solution of fresh water, fresh water and fresh water. The results were added to the tissue culture solution of fresh water and fresh water, and the results were added to the tissue culture solution of fresh water and fresh water. In vivo, ANP, CNP1, seawater, fresh water, fresh water, At the same time, NP ACTH was added to improve the energy production of CNP-1, seawater, ACTH, CNP-4, fresh water and fresh water, respectively. Change the ANG, AM, etc. ANG class 4 (ANGI, ANGII, Ang III Ang IV) has been found to be present in the blood, to detect the presence of acid in the blood, to detect the presence of acid in the blood, to detect the presence of N-ANGII in the blood, to detect the presence of acid in the blood, to detect the presence of acid in the blood, and to express D-ANG in the blood. The results showed that the secretion of N-ANGII in fresh water was 3 times higher than that in ANG. The secretion of N-ANGII was 3 times higher than that in fresh water. ANG-III "N-ANGII" to the same extent. The secretion of AM1, AM2, and AM5 was enhanced by microsomal stimulation. The results of this study are as follows: the results of this study, the results of this study. The ELISA method was confirmed by ACTH, and the pituitary gland was confirmed by the NP method. The pituitary gland was secreted by the ACTH.

项目成果

ウナギ間腎におけるナトリウム利尿ペプチド(NPファミリー)のコルチゾル分泌調節

鳗鱼肾间钠尿肽（NP家族）对皮质醇分泌的调节

• 发表时间: 2008
• 作者: Masatoshi Nakamura;Hiroaki Urabe;Akira Uchiyama;Takaaki Umedu;Teruo Higashino;内山 彰;内山 彰;Akira Uchiyama;Ayano Kagami;加々美 綾乃;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池麻希子;Makiko Kikuchi;菊池麻希子;Makiko Kikuchi;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池 麻希子;Kenji Asano;Miyako Ueguchi-Tanaka;Ko Hirano;Tory Chhun;Yoko Hattor;浅野賢治;浅野賢治;浅野 賢治;Albert Ventura;Albert Ventura
• 通讯作者: Albert Ventura

NPs interact with ACTH for cortisol secretion in the eel interrenal

NPs 与 ACTH 相互作用，促进鳗鱼肾内皮质醇的分泌

• 发表时间: 2009
• 作者: Masatoshi Nakamura;Hiroaki Urabe;Akira Uchiyama;Takaaki Umedu;Teruo Higashino;内山 彰;内山 彰;Akira Uchiyama;Ayano Kagami;加々美 綾乃;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池麻希子;Makiko Kikuchi;菊池麻希子;Makiko Kikuchi;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池 麻希子;Kenji Asano;Miyako Ueguchi-Tanaka;Ko Hirano;Tory Chhun;Yoko Hattor;浅野賢治;浅野賢治;浅野 賢治;Albert Ventura
• 通讯作者: Albert Ventura

An overview of osmoregulation

渗透调节概述

• 发表时间: 2008
• 作者: Masatoshi Nakamura;Hiroaki Urabe;Akira Uchiyama;Takaaki Umedu;Teruo Higashino;内山 彰;内山 彰;Akira Uchiyama;Ayano Kagami;加々美 綾乃;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池麻希子;Makiko Kikuchi;菊池麻希子;Makiko Kikuchi;Makiko Kikuchi;Makiko Kikuchi;菊池 麻希子;菊池 麻希子;菊池 麻希子;Kenji Asano;Miyako Ueguchi-Tanaka;Ko Hirano;Tory Chhun;Yoko Hattor;浅野賢治;浅野賢治;浅野 賢治;Albert Ventura;Albert Ventura;上口 美弥子;Albert Ventura
• 通讯作者: Albert Ventura