Novel drug delivery system using thermo-reversible polymer for brain tumor

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MG132, a proteasome-inhibitor, has been shown to induce apoptosis of experimental malignant cells in vitro. However, its systemic toxicity prevents further use in clinical settings. To circumvent this toxicity, MG132 can be delivered directly to the tumor. We tested the efficacy of MG132 incorporated into thermoreversible gelation polymer (TGP) for treating experimental glioma cell lines were measured with MTT-assays. As a caspase activity assay, the activities for caspases 3, 8 and 9 were measured in a CytoFluor Multiwell Plate Reader. A significant increase in cleavage activities of caspases 3, 9, and 8 detected in caspase cleavage assay results was observed in both cell lines. MG132 exhibits potent cytotoxic-activity against U87MG and T98G in vitro, it can be efficiently incorporated and delivered using TGP.