Development of three types of prostatitis model in rats

三种大鼠前列腺炎模型的建立

• 批准号: 18591780
• 负责人: MATSUMOTO Tetsuro
• 金额: $ 2.57万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591780/
• 关键词: prostate; prostatitis; C-fiber; voiding dysfunction; cytokine; inflammation; pelvic pain; ラット; 肥満細胞; 蓄尿機能; 膀胱

Prostatitis is a syndrome characterized by pelvic pain and voiding symptoms, which is poorly defined, poorly understood, poorly treated and bothersome. Prostatitis is a common cause of visits to primary care physicians and urologists. However, the pathogenesis is largely unknown and the effective treatment remains to be established. We believe that it is necessary to develop new strategy such as using animal model for prostatitis. At present, there are no reliable animal models available for prostatitis. In the present study, we developed three different animal models for prostatitis. For endocrine type, we injected 17 beta-estradiol (E2; 250 microgram/kg. subcutaneously) or vehicle into rats for 30 days. For infections type, we injected lipopolysaccharide (LPS) into rat prostate. For non-specific inflammation type, we injected HC1 into rat prostate. These three animal models demonstrated inflammation in the prostate and decrease intercontraction of the bladder in cystometrogram. The present three models demonstrate histological and physiological features which are similar to prostatitis in human. These animal models could be useful for studying the pathogenesis of prostate.

前列腺炎是一种以盆腔疼痛和排尿症状为特征的综合征，其定义不清，认识不清，治疗不佳，令人烦恼。前列腺炎是一个常见的原因访问初级保健医生和泌尿科医生。然而，其发病机制在很大程度上是未知的，有效的治疗方法仍有待建立。我们认为，有必要开发新的策略，如使用动物模型治疗前列腺炎。目前尚无可靠的前列腺炎动物模型。在本研究中，我们建立了三种不同的前列腺炎动物模型。对于内分泌型，我们注射了17 β-雌二醇（E2; 250微克/kg。皮下）或媒介物注射到大鼠中30天。感染型采用前列腺内注射脂多糖（LPS）。对于非特异性炎症类型，我们将HCl注入大鼠前列腺。这三种动物模型均显示前列腺炎症，膀胱测压显示膀胱内收缩减少。这三种模型均具有与人类前列腺炎相似的组织学和生理学特征。这些动物模型为研究前列腺疾病的发病机制提供了有用的动物模型。

项目成果

Up-regulation of interleukin-6 gene expression in cyclophosphamide-induced cystitis in mice : An in situ hybridization histochemical study.

环磷酰胺诱导的小鼠膀胱炎中白细胞介素 6 基因表达的上调：原位杂交组织化学研究。

• 发表时间: 2006
• 期刊: Internatonal Journal of Urology 13(10)
• 作者: Nishii H;Nomura M;Fujimoto N;Matsumoto T
• 通讯作者: Matsumoto T

Up-regulation of galanin and corticotropin-releasing hormone mRNAs in the key hypothalamic and amygdaloid nuclei in a mouse model of viseral pain

内脏疼痛小鼠模型中关键下丘脑和杏仁核中甘丙肽和促肾上腺皮质激素释放激素 mRNA 的上调

• 发表时间: 2007
• 期刊: Regulatory peptides (in press)
• 作者: Nishii H;Nomura M;Aono H;Fujimoto N;Matsumoto T.
• 通讯作者: Matsumoto T.

Expression of Neuropeptide Y gene in mouse testes during testicular development.

睾丸发育过程中小鼠睾丸中神经肽 Y 基因的表达。

• 发表时间: 2006
• 期刊: Asian Journal of Andrology 8(4)
• 作者: Terado M;Nomura M;Mineta K;Fujimoto N;Matsumoto T
• 通讯作者: Matsumoto T

Prostate cancer cells increase androgen sensitivity by increase in nuclear androgen receptor and androgen receptor caoactivators; a possible mechanism of hormone-resistance of prostate cancer cells.

前列腺癌细胞通过增加核雄激素受体和雄激素受体曹激活剂来增加雄激素敏感性；

• 发表时间: 2007
• 期刊: Cancer Investigation 25(1)
• 作者: Fujimoto N;Miyamoto H;Mizokami A;Harada S;Nomura M;Ueta Y;Sasaguri T;Matsumoto T.
• 通讯作者: Matsumoto T.