Identification and characterization of the genes responsible for the development of uterine cervical carcinoma : towards the molecular targetting.
负责宫颈癌发生的基因的鉴定和表征:走向分子靶向。
基本信息
- 批准号:18591832
- 负责人:
- 金额:$ 2.49万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
(1) Identification of pathways of cervical carcinogenesisCervical squamous cell carcinoma has been supposed to derive from reserve cells in squamo-columnar junction and develop stepwise through mild, moderate and severe dysplasia. Recent studies showed that type of HPV in severe dysplasia was different from that of mild dysplasia, implying that severe dysplasia does not necessarily develop through mild dysplasia. In our current study, we clarified that atypical immature metaplasia is formed by infection of malignant types of HPV, and that a new pathway from atypical immature metaplasia to severe dysplasia does exist.Adenosquamous carcinoma occupies about 1% of the uterine cervical carcinomas. Adenocarcinoma- and squamous cell carcinoma-parts are concurrently detected in adenosquamous carcinoma, however, it has been still unclear whether those two parts are formed separately form different original cells or develop from a single origin differentiating two cell types. We demonstrated ade … More nosquamous carcinoma of the uterine cervix was demonstrated to be a combination tumor originating from a single Stem cell by clonality analysis.(2) Identification of the genes regulating progression of cervical carcinomaThirty-two genes were identified as the genes responsible for development and progression of cervical carcinoma by DNA microarray analysis. Among them, Igfbp5 was shown to be significantly down-regulated from an early step of cervical carcinogenesis. Moreover, point mutation or amplification of PIK3CA gene was identified in 25% of cervical carcinoma cases. Inactivation of RUNX3 genes occurred in about 50% of uterine carcinoma. We also showed that B4-H7, a novel gene responsible for the development of ovarian cancer, plays a role in uterine carcinoma.(3) Analysis of epithelial-stromal interaction of cervical carcinomaWe demonstrated, in vitro and in vivo, that HB-EGF secreted by the stroma of cervical carcinoma promotes proliferation of carcinoma cells by paracrine mechanism. Less
(1)宫颈鳞癌发生途径的研究宫颈鳞状细胞癌起源于鳞状-柱状交界处的储备细胞,通过轻、中、重度不典型增生逐步发展。最近的研究表明,重度不典型增生的HPV类型与轻度不典型增生的HPV类型不同,提示重度不典型增生并不一定通过轻度不典型增生发展。在我们目前的研究中,我们阐明了非典型未成熟化生是由恶性类型的HPV感染形成的,并且确实存在从非典型未成熟化生到重度不典型增生的新途径。腺鳞癌中同时检测到腺癌和鳞癌两个部分,然而,这两个部分是由不同的原始细胞单独形成的,还是从同一来源分化成两种细胞类型仍不清楚。我们演示了ADE…克隆性分析进一步证实宫颈非鳞癌是来源于单个干细胞的复合瘤。(2)宫颈癌进展调控基因的鉴定DNA微阵列分析发现32个基因是宫颈癌发生发展的相关基因。其中,在宫颈癌发生的早期,Igfbp5的表达显著下调。此外,25%的宫颈癌组织中存在PIK3CA基因点突变或扩增。RUNX3基因失活发生在约50%的子宫癌中。我们还发现了与卵巢癌发生发展相关的新基因B4-H7在子宫癌中的作用。(3)宫颈癌上皮-间质相互作用的分析我们在体内外证实了子宫颈癌间质分泌的HB-EGF通过旁分泌机制促进癌细胞的增殖。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
子宮頸部悪性腫瘍におけるPIK3CA遺伝子変異の検討
宫颈恶性肿瘤PIK3CA基因突变检测
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:三宅貴仁;吉野潔;宇垣弘美;金雅子;藤原和子;宮武崇;倉垣千恵;藤田征巳;榎本隆之;木村正
- 通讯作者:木村正
マウスin vivoにおける子宮頸癌細胞株と間質繊維芽細胞の共培養解析
小鼠体内宫颈癌细胞系与基质成纤维细胞的共培养分析
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:村田 卓也;榎本隆之;藤田征巳;東千尋;木村 正
- 通讯作者:木村 正
Frequent Inctivation of RUNX3 in Endometrial Carcinoma
子宫内膜癌中 RUNX3 的频繁激活
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Yoshizaki T;Enomoto T;Fujita M;Ueda Y;Miyatake T;Fujiwara K;Miyake T;Kimura T;Yoshino K;Kimura T
- 通讯作者:Kimura T
Immunohistochemical Validation of B7-H4(D0-0110) as a Biomarker of Ovarian Cancer : Correlation with CA-125
B7-H4(D0-0110) 作为卵巢癌生物标志物的免疫组织化学验证:与 CA-125 的相关性
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Takashi;Miyatake;Kenneth;R.;Shroyer
- 通讯作者:Shroyer
Immunohistochemical Validation of B7-H4(DO-O110)as a Biomarker of Ovarian Cancer: Correlation with CA-125
B7-H4(DO-O110) 作为卵巢癌生物标志物的免疫组织化学验证:与 CA-125 的相关性
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Takashi Miyatake;Kenneth R. Shroyer
- 通讯作者:Kenneth R. Shroyer
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ENOMOTO Takayuki其他文献
ENOMOTO Takayuki的其他文献
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{{ truncateString('ENOMOTO Takayuki', 18)}}的其他基金
Elucidation of pathogenesis of endometriosis and endometriosis-associated ovarian cancer based on genomic analyses for normal endometrium
基于正常子宫内膜的基因组分析阐明子宫内膜异位症和子宫内膜异位症相关卵巢癌的发病机制
- 批准号:
20H03821 - 财政年份:2020
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identification of molecular characteristics in serous carcinoma of Mullerian origin
苗勒管起源浆液性癌分子特征的鉴定
- 批准号:
25293338 - 财政年份:2013
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Targeting annexin A4 to counteract chemoresistance in Gynecologic Cancer
靶向膜联蛋白 A4 抵消妇科癌症的化疗耐药性
- 批准号:
22591854 - 财政年份:2010
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of TSC403/DC-Lamp gene in cervical carcinogenesis
TSC403/DC-Lamp基因在宫颈癌发生中的作用
- 批准号:
16591656 - 财政年份:2004
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification and clinical Application of the Genes Associated with Carc nogenesis of the Uterine Cervix
宫颈癌发生相关基因的鉴定及临床应用
- 批准号:
14571561 - 财政年份:2002
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Histogenesis of carcinosarcoma of the uterine corpus
子宫体癌肉瘤的组织发生
- 批准号:
09671678 - 财政年份:1997
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer
LAMP3/TSC403/DC-LAMP过表达促进宫颈癌转移
- 批准号:
17591736 - 财政年份:2005
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of TSC403/DC-Lamp gene in cervical carcinogenesis
TSC403/DC-Lamp基因在宫颈癌发生中的作用
- 批准号:
16591656 - 财政年份:2004
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)