Identification of genes associated with genesis of epithelial ovarian Identification of genes associated with genesis of epithelial ovarian cancer using non-tumorigenic immortalized ovarian epithelial cell lines

鉴定与上皮性卵巢发生相关的基因 使用非致瘤性永生化卵巢上皮细胞系鉴定与上皮性卵巢癌发生相关的基因

• 批准号: 18591848
• 负责人: TAKANO Hirokuni
• 金额: $ 2.6万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591848/
• 关键词: 卵巣癌; 封入嚢腫; MET; ゲノム発現解析; 不死化細胞; マイクロアレイ; 正常卵巣上皮不死化細胞; 正常卵巣上皮不死化細胞株

卵巣癌発生に関与する遺伝子の検索のため、正常卵巣上皮不死化細胞株(IOSEC)を6株樹立した。正常卵巣上皮(OSE)、封入嚢腫(IC)、樹立したIOSECを用いて,種々の抗原マーカーの発現を免疫組織学的に検討し、ICはMenchymal to Epithelial Transition(MET)過程にあることが示唆された。さらにIOSECとそのprimary culture(PC)細胞間で発現が異なる遺伝子を包括的ヒトゲノム発現解析によりスクリーニングし、卵巣癌発生に関する遺伝子を104種類に絞り込んだ。

6 normal oocyte epithelial cells (IOSEC) were established. Normal oocyte epithelium (OSE), encapsulated cyst (IC), and established IOSEC were used in the study of the development of seed antigens and in the study of immunohistologic changes in IC and Menchymal to Epithelial Transition(MET). In addition, IOSEC and the primary culture(PC) cell to cell development of different genes, including the analysis of gene development, egg cancer development related to the gene 104 species.

项目成果

Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation

• DOI: 10.3892/ijmm_00000121
• 发表时间: 2009-02-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
• 影响因子: 5.4
• 作者: Omi, Hiroko;Okamoto, Aikou;Tanaka, Tadao
• 通讯作者: Tanaka, Tadao

IGFBP1 and Follistatin-like 3 genes are significantly up-regulated in expression profiles of the IUGR

IGFBP1 和 Follistatin-like 3 基因在 IUGR 的表达谱中显着上调

• 发表时间: 2006
• 期刊: Placenta. 27
• 作者: Okamoto A;Endo H;Kalionis B;Shinya M;Saito M;Nikaido T;Tanaka T
• 通讯作者: Tanaka T

Establishment of an immortalized human extravious trophoblast cell line by retroviral infection of HPV E6/E7 and hTERT. International College of Surgeons,

通过 HPV E6/E7 和 hTERT 逆转录病毒感染建立永生化人外源滋养层细胞系。

• 发表时间: 2007
• 作者: Omi H;Okamoto A;Nikaido T;Saito M;Umehara N;Kawaguchi R;Ikeya M;Sugiura K;Kiyono T;Tanaka T
• 通讯作者: Tanaka T

Molecular approach for individualized therapy in ovarian cancer.

卵巢癌个体化治疗的分子方法。

• 发表时间: 2007
• 作者: Aikou Okamoto;Nikaido T;Urashima M;Takao M;0mi H;Okamoto S;Yanaihara N;Takakura S;OchiaiK;Tanaka T.
• 通讯作者: Tanaka T.

Mesenchymal to epithelial transition in the human ovarian surface epithelium focusing on inclusion cysts

• DOI: 10.3892/or_00000343
• 发表时间: 2009-05-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
• 作者: Okamoto, Sanshiro;Okamoto, Aikou;Tanaka, Tadao
• 通讯作者: Tanaka, Tadao