The Meanings of Galanin and Galanin Reseptors In Head and Neck Cancer

甘丙肽和甘丙肽受体在头颈癌中的意义

• 批准号: 18591903
• 负责人: OGAWA Tetsuya
• 金额: $ 2.05万
• 依托单位: Aichi Medical University (2007)Aichi Cancer Center Research Institute (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591903/
• 关键词: Head and Neck Cancer; Gprotein coupled receptor; signal transduction; oncogene; tumor suppressor gene; 癌遺伝子; 癌抑制遺伝子; 頭頚部癌

This research was done both in Aichi Cancer Center Hospital and Aichi Medical University because the head investigator changed his position. During 2006 to 2007, the expressions of Galanin receptor 1 and Galanin receptor 2 were examined using immunohistochemical technique. In 2006, samples were selected from those operated for tongue cancer at Aichi Cancer Center Hospital. On 2007, the samples of many other head and neck cancers were selected from patients operated at Aichi Medical University. (Results of 2006 research) Tongue cancer samples were used. The antibodies used were made by Gene Tex. The expression of Galanin receptor 1 was seen in normal tissue. However, in the tumor tissue, the expression of Galanin receptor 1 was rarely seen. For Galanin receptor 2, the expression was not seen in either normal or tumor tissue. (Results of 2007) The research was done in Aichi Medical University. The antibodies used were made by Alpha Diagnostic. In the normal tissue, both the expression of … More Galanin receptor 1 and 2 were seen. However, in the tumor tissue, the expression of Galanin receptor 1 was decreased. The expression Galanin receptor 2 was very strong. In addition, the parotid malignant tumor specimen was used and examined. The expressions of Galanin receptor 1 and 2 were confirmed in the parotid tissue for the first time. We focused on a mixed case sample of a less malignant lesion and a highly malignant lesion. In the less malignant lesion, the expression of Galanin receptor 1 was higher than that of Galanin receptor 2. On the other hand, in the highly malignant lesion, the expression of Galanin receptor 1 was lower than that of Galanin receptor 2.(Discussion) We originally assumed that Galanin receptor 1 worked as a tumor suppressor gene and Galanin receptor 2 as an oncogene. This difference and distribution derive from the signal transduction differences from the different G-protein coupling. From the results of 2007 research, Galanin receptor 1 will be able to work as a tumor suppressor gene, and Galanin receptor 2 as an oncogene. It seems that a further examination is necessary in the future. Less

这项研究是在爱知肿瘤中心医院和爱知医科大学都进行的，因为首席研究员换了位置。2006~2007年采用免疫组织化学方法检测甘丙肽受体1和甘丙肽受体2的表达。2006年，样本是从爱知肿瘤中心医院接受舌癌手术的患者中挑选出来的。2007年，许多其他头颈癌的样本是从爱知医科大学手术的患者中挑选出来的。(2006年研究结果)使用的是舌癌样本。使用的抗体是由基因特克斯公司制造的。Galanin受体1在正常组织中有表达。然而，在肿瘤组织中，Galanin受体1的表达很少见。对于Galanin受体2，无论是在正常组织还是在肿瘤组织中都没有表达。(2007年结果)研究是在爱知医科大学完成的。所使用的抗体是由阿尔法诊断公司制造的。在正常组织中，…的表达更多的Galanin受体1和2可见。但在肿瘤组织中，Galanin受体1的表达减少。Galanin受体2的表达很强。此外，还对腮腺恶性肿瘤标本进行了检测。首次证实Galanin受体1和2在腮腺组织中有表达。我们专注于一个混合的病例样本，其中包括一个恶性程度较低的病变和一个恶性程度较高的病变。在恶性程度较低的病变中，Galanin受体1的表达高于Galanin受体2，而在高度恶性病变中，Galanin受体1的表达低于Galanin受体2。(讨论)我们最初假设Galanin受体1作为抑癌基因，Galanin受体2作为癌基因。这种差异和分布源于不同的G蛋白偶联导致的信号转导差异。从2007年的研究结果来看，Galanin受体1将能够作为肿瘤抑制基因发挥作用，Galanin受体2将作为癌基因发挥作用。看来未来有必要进行进一步的检查。较少