Basic study for dinical application of a novel biomedical material "Anodized titanium plate

在这项研究中，我们检测了几种溶液阳极化的钛板对致龋性革兰氏阳性菌变形链球菌的抑菌效果。考察了NaC_1、Na_2CO_3、MgSO_4、KCl、MgC_2、CH_3COOK作为阳极氧化液的性能。当使用Na2CO3、MgSO4和CH3COOK时，没有观察到抑菌效果，而NaCl1、KCl1和MgCl2则认为氯等卤素对抑菌效果是绝对必要的。对变形链球菌、戈登链球菌、远缘链球菌、金黄色葡萄球菌、化脓性链球菌、白色念珠菌、光滑念珠菌的抑菌试验表明，对革兰氏阳性和革兰氏阴性菌均有效，但对真菌如C、白色念珠菌的作用较差。在无菌水中浸泡2周后，阳极氧化钛板的抑菌效果没有下降，而在磷酸盐缓冲液(PBS)或RPMI1640介质中浸泡后，抑菌效果显著降低。这些结果表明，抗菌剂的抑菌作用是基于pH的。这可能是合理的含氯酸性物质，如HClO从阳极氧化钛板表面释放出来。这些结果表明，这种新型的氯化钠阳极氧化钛板可以有效地防止细菌的附着，并有效地附着在成骨细胞等宿主细胞上。这种处理对于植入物使用可能是非常有效的，因为在这种情况下，我们不必重复使用这种材料。经氯化钠氧化的种植体可能有效地减少了口腔和/或全身细菌感染的机会。

In this study, we examined the anti-bacterial effect of titanium plates, anodized by several solutions on cariogenic gram positive bacteria Streptococcus mutans. NaC1, Na_2CO_3, MgSO_4, KC1, MgC1_2, CH_3COOK were examined as anodization solution. When using Na_2CO_3, MgSO_4, and CH_3COOK, anti-bacterial effect was not observed, in contrast, NaC1, KC1, and MgC1_2, were figured out that halogen such as Cl was absolutely imperative for the anti-bacterial effect. Anti-bacterial examination against Streptococcus mutans, Streptococcus gordonii, Streptococcus sobrinus, Escherichi Staphylococcus aureus, Streptococcus pyogenes, Candida albicans, and Candida glabrata showed that it is very effective against bacteria both gram positive and gram negative, however, the effect against fungus such as C, albicans, were less effective. No reduction of the anti-bacterial effect of the anodized titanium plate was observed after immersion in sterile water for 2 weeks, however, after immersion in phosphate buffer saline(PBS) or RPMI1640 medium, the anti-bacterial effect was remarkably reduced. These results suggest that the anti-bacterial effect is based on pH. It might be reasonable that something acid containing Cl, such as HClO was released from the surface of anodized titanium plate. These results indicate that this novel titanium plate anodized by NaCl is effective to prevent the attachment of bacteria and effective to attach the host cells such as osteoblast. This disposition might be very effective for implant use as in this case, we do not have to reuse this material. It is possible that the chance of infection by oral and/or general bacteria is effectively reduced with the implant anodized by NaCl.