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Evaluation of antibacterial activity and shear bond strength of 4-META/MMA-TBB resin containing catechin agent.

含儿茶素剂的4-META/MMA-TBB树脂的抗菌活性和剪切粘合强度评价。

基本信息

批准号：
18592251
负责人：
SAITO Kayo
金额：
$ 2.62万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592251/
关键词：
green-tea catechin 4-META MMA-TBB resin antibacterial activity sher bond strength

项目摘要

The purpose of this study was to produce an antibacterial adhesive for orthodontic bonding without compromising the mechanical property. We added green tea catechin to the Superbond C&B (4-META/MMA-TBB)resin that exhibits a strong bonding strength between enamel and bracket. The catechins used in this study were(+)-catechin, (-)-epicatechin (EC), (-)-epigallocatechin(EGC), (-)-epicatechin gallate(ECg), and (-)-epigallocatechin gallate(EGCg). Each catechin concentrations in the catechin-composites were 10.0%(wt/wt). The sample disks of uniform size(8.0mm diameter ×2.0mm thick)were made from all the catechin-composites using custom-made molds. Oral cariogenic streptococci, Streptococcus mutans NCTC10449 was used as the test bacteria. The disks were put in the bacteria infusion at 37℃ for 24h. They were divided into a disk and bacteria infusion. Each bacteria were prepared in TH buffer. The mitis salivarius agar were cultured anaerobically at 37℃ for 48h, and the number of colony forming unite(CFU) were counted.As a result of bacteria stuck to the disk, The CFU decreased with (+)-catechin, EGC, ECg, EGCg composite. As a result of bacteria infusion that catechin released, The catechin-composite samols showed significant antibacterial activity compared with the control.The catechin-composites were used to bond metal brackets to self-etching primed enamel surface of human premolars. The shear bond strengths were measured after immersion in water at 37℃ for 24h.As a result of shear bond strengths, A sign cant difference was found between the control composite and EGCg and ECg composite. However, the bond strength was more strong than 10MPaThese results confirmed that clinically acceptable bond strength was preserved in 4-META/MMA-TBB resin with added green tea catechin.

本研究的目的是生产一种用于正畸粘合的抗菌粘合剂，而不影响机械性能。我们在 Superbond C&B (4-META/MMA-TBB) 树脂中添加了绿茶儿茶素，使牙釉质和托槽之间具有很强的粘合强度。本研究中使用的儿茶素为(+)-儿茶素、(-)-表儿茶素(EC)、(-)-表没食子儿茶素(EGC)、(-)-表儿茶素没食子酸酯(ECg)和(-)-表没食子儿茶素没食子酸酯(EGCg)。儿茶素复合物中的各儿茶素浓度均为10.0%(wt/wt)。尺寸均匀（直径8.0mm×厚2.0mm）的样品盘由所有儿茶素复合材料使用定制模具制成。试验菌采用口腔致龋链球菌、变形链球菌NCTC10449。将圆片放入菌液中37℃培养24h。它们分为盘和细菌输液。每种细菌均在 TH 缓冲液中制备。将mitis salivarius琼脂在37℃厌氧培养48h，计数菌落形成单位(CFU)数。由于细菌粘在盘上，(+)-儿茶素、EGC、ECg、EGCg复合物导致CFU下降。由于儿茶素释放的细菌输注，儿茶素复合物 samol 与对照相比显示出显着的抗菌活性。儿茶素复合物用于将金属托槽粘合到人前磨牙的自酸蚀底釉质表面。在37℃水中浸泡24h后测量剪切粘合强度。剪切粘合强度结果显示，对照复合材料与EGCg和ECg复合材料之间存在符号斜差。然而，粘合强度高于10MPa。这些结果证实，添加绿茶儿茶素的4-META/MMA-TBB树脂保留了临床可接受的粘合强度。