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Identifying candidate genes involved with reduced masticatory sensory input in senescence accelerated mouse prone 8 hippocampus

鉴定与加速衰老的小鼠 8 海马体咀嚼感觉输入减少相关的候选基因

基本信息

批准号：
18592254
负责人：
SASAGURI Kenichi
金额：
$ 2.49万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Many of experimental procedures for examining the influence of reduced masticatory input on the brain function often use powdery food that crushes it with the solid food usually used to feed. In this research, we have originally developed liquid diet foods for changing the more differential masticatory situation through it was a calories and compositions similar to the usual used solid food in 2006. And then, the liquid diet food was fed to the senescence accelerated mouse prone 8 in 2007. However, the reason was uncertain that a lot of experimental animals had died; the accomplishment of the experimental procedure became difficult. Then, we have examined the gene expression by using microarry system in rat hippocampus when the experimental animals expose to acute restraint stress and displayed biting behavior during the same task referring to the Kim's report (J.Neuroscience 2005; 25(6):1532-9.). As a result, we were able to pick up about 30 genes, such as heat shock protein, toll-like receptor 3, that showed the appearance twice or more changed. Simultaneously, we have also picked up about 70 genes as a gene that admitted 1/2 times or less than it. In Morris water maze analysis, spatial memory and learning activity of biting group of ddy mice were indicated more recovery the ability compared with the stress only group. Moreover, the expression of Fos protein, which generally used as a maker for neuronal activity, have decreased on CA1, CA3 and DG of rat hippocampus in biting group compared with stress only group in the immunohistochemistry study.These findings possibility suggested that biting activity during the severe acute stress was attenuated impaired hippocampus function.

许多研究咀嚼输入减少对大脑功能影响的实验程序经常使用粉末状食物，这些食物与通常用于喂养的固体食物一起压碎。在这项研究中，我们最初开发了液体饮食食品，以改变更有差异的咀嚼情况，通过它是一种热量和成分类似于2006年通常使用的固体食品。然后，于2007年对加速衰老的小鼠prone 8饲喂流质饮食食物。然而，原因不明，大量实验动物死亡，实验程序的完成变得困难。然后，参考Kim的报告（J.Neuroscience 2005; 25（6）：1532-9.），我们通过使用微阵列系统检测了当实验动物暴露于急性束缚应激并在同一任务期间表现出咬行为时大鼠海马中的基因表达。结果，我们能够挑选出大约30个基因，如热休克蛋白，Toll样受体3，这些基因显示了两次或更多次的外观变化。在Morris水迷宫分析中，与单纯应激组相比，咬食组ddy小鼠的空间记忆和学习活动有更强的恢复能力。免疫组化结果显示，与单纯应激组相比，咬合组大鼠海马CA 1、CA 3和DG区Fos蛋白表达明显减少，提示重度急性应激时咬合活动减弱，海马功能受损。