In vivo patch-clamp analysis of developmental changes in the spinal nociceptive circuit.

脊髓伤害性回路发育变化的体内膜片钳分析。

基本信息

  • 批准号:
    18613009
  • 负责人:
  • 金额:
    $ 2.62万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

Developmental changes in the spinal nociceptive synaptic transmission were examined using slice and in vivo patch-clamp recordings. In transverse spinal cord slices of young and adult rats at lumbar spinal levels with an attached dorsal root, electrical dorsal root stimulation was applied to record the evoked-synaptic responses in substantia gelatinosa (SG, lamina II of the spinal cord)and deep lamina (lamina III-IV) neurons. In the in vivo patch-clamp analysis, patch electrodes were inserted into the SG at the spinal level of L3-L5 after a thoracolumbar laminectomy was performed under urethane anesthesia. Whole-cell recordings were obtained from SG neurons, and then modality-dependent synaptic responses evoked by cutaneous natural mechanical stimuli applied to the ipsilateral hind limb were recorded. In adult rats, monosynaptic Aβ and C fiber-evoked EPSCs were elicited in SG neurons. Additionally, monosynapitc Aβ fiber-evoked EPSCs were evoked in the deeper lamina neurons but not in SG neurons. In young rats, on the other hand, monosynaptic Aβ fiber-evoked EPSCs were observed in SG neurons in addition to Aβ and C fiber-evoked responses. In the in vivo patch-clamp analysis, pinch stimulation elicited a barrage of EPSCs in SG neurons in young and adult rats. Even in SG neurons that received synaptic inputs from only Aβ fibers, pinch stimulation evoked a barrage of EPSCs. Most deep neurons in adult rats responded to touch stimulation. The present results suggest that Aβ fibers, in addition to Aδ and C fibers, convey noxious mechanical information to SG neurons in young rats. In adult rats with further development of neuronal circuits in the spinal dorsal horn, innocuous mechanical synaptic responses are mediated only through Aβ fibers in deep lamina neurons.
使用切片和体内膜片钳记录检查脊髓伤害性突触传递的发育变化。在横向脊髓切片的年轻和成年大鼠在腰脊髓水平与一个附加的背根,电背根刺激被施加到记录胶状质(SG,脊髓的第二层)和深层(第三层-第四层)神经元的诱发突触反应。在体内膜片钳分析中,在乌拉坦麻醉下进行胸腰椎椎板切除术后,将贴片电极插入L3-L5脊柱水平的SG中。从SG神经元获得全细胞记录,然后记录施加到同侧后肢的皮肤自然机械刺激诱发的模态依赖性突触反应。在成年大鼠,SG神经元可诱发单突触Aβ和C纤维诱发的EPSC。此外,单突触Aβ纤维诱发的EPSC在更深的板层神经元中被诱发,而在SG神经元中不被诱发。另一方面,在年轻大鼠中,除了Aβ和C纤维诱发的反应外,还在SG神经元中观察到单突触Aβ纤维诱发的EPSC。在体内膜片钳分析中,捏刺激在年轻和成年大鼠的SG神经元中引起了大量的EPSC。即使在只接受来自Aβ纤维的突触输入的SG神经元中,捏刺激也诱发了大量的EPSC。成年大鼠的大多数深层神经元对触摸刺激有反应。结果提示,幼年大鼠SG神经元除了接受Aδ和C纤维的传递外,还接受Aβ纤维的传递。在脊髓背角神经回路进一步发育的成年大鼠中,无害的机械突触反应仅通过深板层神经元中的Aβ纤维介导。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Selective modulation of excitatory synaptic transmission in excitatory interneurons in the adult rat spinal dorsal horn through TRPA1 agonist-sensitive C fibres
通过 TRPA1 激动剂敏感 C 纤维选择性调节成年大鼠脊髓背角兴奋性中间神经元的兴奋性突触传递
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Uta D.;et. al.
  • 通讯作者:
    et. al.
Activation of TRPA1 reduces C afferent-evoked excitatory synaptic transmission but enhances the miniature release in substantia gelatinosa of the adult rat spinal cord
TRPA1 的激活减少了 C 传入诱发的兴奋性突触传递,但增强了成年大鼠脊髓胶质质的微型释放
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Uta D.;et. al.
  • 通讯作者:
    et. al.
Involvement of Kv channel subtypes on GABA release in mechanically dissociated neurons from the rat substantia nigra.
Kv 通道亚型对大鼠黑质机械分离神经元 GABA 释放的参与。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Shimada H.;et. al.
  • 通讯作者:
    et. al.
Patch-clamp analysis : advanced techniques
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    W. Walz;A. Boulton;G. Baker
  • 通讯作者:
    W. Walz;A. Boulton;G. Baker
Increased mechanical synaptic inputs o the rat spinal dorsal horn in vivo following inflammation.
炎症后大鼠脊髓背角体内机械突触输入增加。
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    H.;Fume;J.;Hachisuka;M.;Yoshimura
  • 通讯作者:
    Yoshimura
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FURUE Hidemasa其他文献

FURUE Hidemasa的其他文献

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{{ truncateString('FURUE Hidemasa', 18)}}的其他基金

In vivo genetic manipulation and analysis of neuronal nociceptive circuits in the brain
大脑神经元伤害性回路的体内基因操作和分析
  • 批准号:
    17H04114
  • 财政年份:
    2017
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of in vivo patch-clamp recording technique from the anterior cingulate cortex
前扣带皮层体内膜片钳记录技术的发展
  • 批准号:
    16K15341
  • 财政年份:
    2016
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Central neuronal mechanisms for descending antinociception and arousal states in the brain
大脑中降低镇痛和唤醒状态的中枢神经元机制
  • 批准号:
    26293132
  • 财政年份:
    2014
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
In vivo patch-clamp analysis of spinal GABA-mediated inhibitory system
脊髓 GABA 介导的抑制系统的体内膜片钳分析
  • 批准号:
    25670289
  • 财政年份:
    2013
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Central modulation of spinal nociceptive synaptic transmission in vivo
体内脊髓伤害性突触传递的中枢调节
  • 批准号:
    23390156
  • 财政年份:
    2011
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
In vivo patch-clamp analysis of plastic changes in descending inhibitory system
下行抑制系统塑性变化的体内膜片钳分析
  • 批准号:
    20602003
  • 财政年份:
    2008
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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