Pore-Size Control and Fictionalization of Nanoporous Membrane Prepared from Block Copolymer Precursor for Medical Device Materials

以聚乙烯（PE）/聚苯乙烯（PS）两嵌段共聚物（PE/PS）为原料，采用酸蚀法在不同条件下制备了不同孔径的纳米多孔膜。扫描探针显微镜（SPM）的观察表明，孔的形态强调与消除无定形PS相的蚀刻时间的延长。然而，进一步的蚀刻破坏了支撑纳米多孔膜的网络几何形状的PE结晶相。对于孔形态的定量分析，通过从一系列扫描探针显微镜图像中提取的高度分布可评价孔径分布。孔径大小可通过酸处理条件控制。用Hep G2细胞进行细胞增殖试验，检测它们的生物学应用。证实了本研究中制备的所有膜的细胞增殖。然而，与具有光滑表面的未蚀刻膜相比，表现出典型纳米多孔形态的膜在通过胰蛋白酶添加和离心进行细胞去除处理后得到较低数量的细胞。这些结果表明对于前一种膜，细胞与膜表面之间的牢固附着。

Nanoporous membranes with different pore dimensions were prepared from a series of polyethylene (PE)/polystyrene (PS) diblock copolymer films by acid etching under various conditions. Scanning probe microscope (SPM) observation indicated that the pore morphology was emphasized by elongation of etching time with elimination of amorphous PS phase. However, further etching destructs the PE crystalline phase which supports the network geometry of nanoporous membrane. For quantitative analyses of pore morphology, pore size distribution was evaluable by the height profiles extracted from a series of the scanning probe microscope images. The pore size was controllable by acid treatment conditions. Their biological applications were examined by cell proliferation tests using Hep G2 cells. Cell proliferation was confirmed for all membranes prepared in this study. However, membranes exhibiting typical nanoporous morphologies gave the lower numbers of cells after cell removal treatment by trypsin addition and centrifugation, compared to those for un-etched membrane having smooth surface. These results indicate the robust attachment between cell and membrane surface for the former membrane.