Analysis of cis-element and trans- factors relating with responsiveness and metamorphosis of insect

与昆虫反应性和变态相关的顺式和反式因子分析

• 批准号: 19380033
• 负责人: KAWASAKI Hideki
• 金额: $ 12.73万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380033/
• 关键词: カイコ; 翅原基; クチクラ蛋白質遺伝子; 遺伝子銃; FTZ-F1; BR-C; エクダイソン; EcRE; 養蚕; 蚕糸

We isolated upstream region of cuticle protein genes BMWCP2 and BMWCP5 and clarified the ecdysone responsive sequence. We constructed the method of the introduction of DNA into wing discs and wing tissues by a gene gun. We clarified that BR-C Z2, which is an early ecdysone responsive gene, bound and activated the promoter activity the BMWCP10. We found EcRE upstream of BMWCP10 and showed the binding of EcR to EcRE by a gel electrophoresis mobility sift assay and a reporter assay. We found that the different transcription factors were expressed in different region of the epidermis.

我们分离了角质层蛋白基因BMWCP 2和BMWCP 5的上游区域，并阐明了蜕皮激素反应序列。建立了基因枪法将DNA导入翅盘和翅组织的方法。我们阐明，BR-C Z2，这是一个早期蜕皮激素反应基因，结合并激活启动子活性的BMWCP 10。我们发现EcRE上游的BMWCP 10和EcR的结合EcRE的凝胶电泳迁移率筛选试验和报告分析。我们发现不同的转录因子在表皮的不同区域表达。

项目成果

SSFTZ-F1 and Broad-Complex positively regulate the transcription of the wing cuticle protein gene, BMWCP5, in wing discs of Bombyx mori

SSFTZ-F1 和 Broad-Complex 正向调节家蚕翅盘中翅角质层蛋白基因 BMWCP5 的转录

• 发表时间: 2009
• 期刊: Insect Biochem. Mol. Biol. 39
• 作者: Wang;Iwanaga;Kawasaki
• 通讯作者: Kawasaki

The genome of a lepidopteran model insect, the silkworm Bombyx mori.Insect Biochem. Mol. Biol.

鳞翅目模式昆虫，家蚕 Bombyx mori 的基因组。Insect Biochem。

• 发表时间: 2008
• 期刊: Xia et al Genome-wide identification of cuticular protein enes in th silkwrm, Bombyx mori. Insect Biochem. Mol. Biol. 38
• 作者: Futahashi;Okamoto;Kawasaki;Zhong;Iwanaga;Mita;Fujiwara.
• 通讯作者: Fujiwara.

The degradation of the basement membrane in the metamorphic or gans during the larval pupal transformation of Bombyx mori

家蚕幼虫蛹转化过程中变态组织基膜的降解

• 发表时间: 2008
• 期刊: J. Insect Biotech. Seric 77
• 作者: Manickam;Iwanaga;Kawasaki
• 通讯作者: Kawasaki

20-hydroxyecdysone and juvenile hormone analogue prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori via Kruppel-homolog 1 and Broad

20-羟基蜕皮酮和保幼激素类似物通过 Kruppel 同源物 1 和 Broad 预防家蚕隐性三蜕突变体的早熟变态

• 发表时间: 2011
• 作者: Hua-Bing Wang;M Moriyama;M Iwanaga;Hi Kawasaki
• 通讯作者: Hi Kawasaki

カイコにおける成虫原基成長因子の発現解析

家蚕成虫盘生长因子的表达分析

• 发表时间: 2009
• 作者: Wang;Iwanaga;Kawasaki;王・作道・川崎・荒木・岩野・岩永・藤本・高田・土田
• 通讯作者: 王・作道・川崎・荒木・岩野・岩永・藤本・高田・土田