Molecular breedling for cold stress tolerant forage grasses

建立了结丝草（Zoysia japonica）快速高效的离体培养体系和以成熟胚为外植体的粒子轰击和农杆菌介导转化新方法。来自蒂莫西的果糖转移酶基因（PpFT1）参与了高聚合果聚糖的合成，通过果聚糖和葡萄糖的积累提高了冷驯化植物的抗冻性。多年生黑麦草CBF基因（LpCBFIVa）的耐寒性随开花时间的延长而增加。

Rapid and efficient in vitro culture system and novel methods for particle bombardment and Agrobacterium-mediated transformation using mature embryos as explants have been established in zoysiagrass (Zoysia japonica). Fructsyltransferase gene from timothy (PpFT1) involving a synthesis of highly polymerized fructan contributed to increase freezing tolerance by accumulation of fructan and glucose in cold acclimated plants. CBF gene (LpCBFIVa) from perennial ryegrass functioned with an increase of freezing tolerance with delaying flower time.