Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation

在本研究中，我们研究了卵母细胞特异性基因Oog1的功能。当合子基因激活时，该基因移位到受精胚胎的核中。为了分析该基因的功能，我们建立了Oog1基因表达缺失的转基因小鼠。此外，我们还研究了Oog1在雌性减数分裂过程中的蛋白表达，发现Oog1蛋白存在于减数分裂前期的卵母核中。在另一个实验中，我们确定了控制基因表达的Oog1的调节区。

In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.