Development of islet isolation technique to minimize tissue injury by applying nanotechnology

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In this study, we attempted to develop islet isolation procedure to improve the quality and quantity of isolated islets by applying nanotechnology especially to islet purification and pancreas dissociation steps. On the islet purification step, we clarified by fluid dynamic analysis the mechanical force, shear, contributes to islet destruction and succeed to improve islet yield by using purification solution with lower viscosity than the standard solution. On the pancreas dissociation step, we succeeded to measure O_2 consumption of pancreas during this step.