Molecule targeted therapy for Cholangiocarcinoma based on the gene profiling of cancer stem cell

基于癌症干细胞基因谱的胆管癌分子靶向治疗

• 批准号: 19390348
• 负责人: NAGINO Masato
• 金额: $ 11.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390348/
• 关键词: 胆管癌幹細胞; 分子標的治療; 肝門部胆管癌

Cancer stem cell, have the ability of differentiation and self-reproduction, is important in carcinogenesis and cancer progression.We analyzed a gene profiling between CD133 positive HuCCT1 and the CD133 negative HuCCT1 by DNA array. As a result, we were not able to find the relationship between CD133 and hepatic stem cell marker, such as CD45, TER, CD49f, and c-Met.And then, we inoculated CD133 positive HuCCT1 and the CD133 negative HuCCT1 in the nude mouse. There was significant difference of carcinogenesis in this model. We considered that this caused in various factors, such as the cancer origin, another cancer stem cell marker except CD133.In addition, we administered Nek2 siRNA in liver metastasis of a nude rat through portal vein using the access port. We recognized decrease of the number of the liver metastasis and the reduction of the tumor volume.We clarified that the drug delivery system of siRNA through the portal vein was effective.

肿瘤干细胞具有分化和自我繁殖的能力，在肿瘤的发生和发展中起着重要作用，我们利用DNA芯片技术对CD 133阳性和CD 133阴性的HuCCT 1进行了基因表达谱分析。结果未发现CD 133与肝干细胞标志物CD 45、TER、CD 49 f、c-Met之间的关系。该模型的致癌性有显著性差异。我们认为这是多种因素造成的，如癌症起源，另一种癌症干细胞标志物，除了CD 133。此外，我们通过门静脉给药Nek 2 siRNA在裸鼠肝转移中使用的接入端口。我们认识到肝转移的数量减少和肿瘤体积缩小，我们阐明了siRNA通过门静脉的药物递送系统是有效的。

项目成果

15-deoxy-delta 12,14-prostaglandin J2 prevents inflammatory response and endothelial cell damage in rats with acute obstructive cholangitis.

• DOI: 10.1152/ajpgi.00233.2009
• 发表时间: 2010
• 期刊: American journal of physiology. Gastrointestinal and liver physiology
• 作者: Katsutaka Watanabe;Y. Yokoyama;T. Kokuryo;K. Kawai;Tomomi Kitagawa;T. Seki;A. Nakagawa;M. Nagino

siRNAを用いた癌分子標的治療のトランスレーショナルリサーチ

使用 siRNA 进行癌症分子靶向治疗的转化研究

• 发表时间: 2010
• 作者: 國料俊男;横山幸浩;梛野正人
• 通讯作者: 梛野正人

Is there any effect of renal failure on the hepatic regeneration capacity following partial hepatectomy in rats?

肾衰竭对大鼠肝部分切除术后肝再生能力有影响吗？

• 发表时间: 2007
• 期刊: Biochem Biophys Res Commun. 352
• 作者: Kawai T;Yokoyama Y;Nagino M;(他2名)
• 通讯作者: (他2名)

Apoptosis induction by antisense oligonucleotides against miR-17-5p and miR-20a in lung cancers overexpressing miR-17-92

• DOI: 10.1038/sj.onc.1210425
• 发表时间: 2007-09-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Matsubara, H.;Takeuchi, T.;Takahashi, T.
• 通讯作者: Takahashi, T.

siRNAを用いた癌分子標的治療のトランス

利用 siRNA 改造癌症分子靶向治疗

• 发表时间: 2010
• 作者: 國料俊男;横山幸浩;梛野正人
• 通讯作者: 梛野正人