Molecular mechanism and physiological function of glutathione-dependent regulation of plastidic aldolase

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Glutathione regulates various aspects of physiological events in plants. In order to understand the mechanism underlying the glutathione-dependent physiological events, we focused on a plastidic aldolase, one of proteins undergoing glutathionylation. There were three genes encoding plastidic fructose-1,6-bisphosphate aldolase in the Arabidopsis genome. The glutathione-binding isozyme FBA1 was quantitatively minor (2% of the total plastid FBA proteins in the leaf) and categorized into a phylogenic branch different from that containing the other two isozymes FBA2 and FBA3, which were conventionally considered to be involved in the Calvin cycle. However, only FBA1 activity was upregulated by glutathione and the decrease in FBA1 increased the sensitivity against light. Taken together, the Calvin cycle involves new isozyme.