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Characterization of dog allergens and their application to develop a control method for dog allergy

狗过敏原的表征及其在开发狗过敏控制方法中的应用

基本信息

批准号：
19580356
负责人：
KAMATA Yoichi
金额：
$ 2.91万
依托单位：
National Institute of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2010
项目状态：
已结题

项目摘要

Human-animal bonds become stronger. Dogs as behalf of pets are tightly associated with human living life. Due to the intimate relationship, human may be sensitized with and exhibit allergic response to dog allergens. To prevent dog allergy and assess the risk of dog allergens, characterization of dog allergens was performed. From the analysis of amino-acid sequence of Can f 1 and Can f 2 (major dog allergens), both allergens revealed the barrel structure and one disulfide bond, which were the charters of popular antigenic proteins. X-ray small angle scattering analysis and circular dichroism photometric method indicated that both allergens were globular and β-sheet-abundant proteins, and showed mono-molecular distribution in water-solutions. Also, both allergens showed the values of molecular weight in solution, which was accordance with those estimated from the amino-acid sequence. Dog albumin (Can f 3) being as one of dog allergens was also examined its allergic cross reactions. The IgE antibody in the serum of dog allergy patients bound to albumin of dog and other animal species. This fact indicates that dog allergens must be recognized as widely distributed risk substances.

人与动物之间的纽带变得更加牢固。狗作为宠物的代表，与人类的生活息息相关。由于狗与人的亲密关系，人可能会对狗的过敏原产生致敏和过敏反应。为了预防狗过敏和评估狗过敏原的风险，对狗过敏原进行了表征。从犬主要过敏原Can f1和Can f2的氨基酸序列分析，两种过敏原均显示出桶状结构和一个二硫键，这是常见的抗原蛋白的特征。x射线小角散射分析和圆二色光度法表明，这两种过敏原均为球状和β-薄片丰富的蛋白质，在水溶液中呈单分子分布。两种过敏原在溶液中的分子量与氨基酸序列的测定结果一致。犬白蛋白（Can f3）作为犬类过敏原之一，对其过敏交叉反应进行了检测。犬过敏患者血清中IgE抗体与犬及其它动物白蛋白结合。这一事实表明，必须认识到狗过敏原是广泛分布的危险物质。