Purification of anterior pituitary cells and its apply to the study of cell to cell interaction

垂体前叶细胞的纯化及其在细胞间相互作用研究中的应用

• 批准号: 19590194
• 负责人: YASHIRO Takashi
• 金额: $ 3万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590194/
• 关键词: 組織学; 解剖学; 細胞・組織; シグナル伝達; 糖鎖; 下垂体; ホルモン; 細胞接着

This study is focused on identifying the mechanisms regulating cell-cell affinities in anterior pituitary gland. In the gland, 5 kinds of hormone-producing cells are distributed in an intriguing manner. For example, LH/FSH cells are often surrounded by prolactin cells, while ACTH cells are located adjacent to GH cells very frequently. Further, folliculostellate cells, which does not produce hormone, have a propensity to adhere each other. Based on these observations, we hypothesized that there is signal transductions between these cell types through the cell adhesions, and live cell purification and identification are crucial to decipher the precise mechanism. Recently, we have established two different methods utilizing lectin and genetically modified S100-b-GFP rats, which allow us to purify and identify live hormone producing cells and live folliculostellate cells from the mixed population of anterior pituitary cells, respectively. The data obtained from two approaches suggested that there are three local regulatory mechanisms in anterior pituitary cells; matricrine (effect of matrix), juxtacrine (effect of cell adhesion molecule), and paracrine (local expression of retinaldehyde dehydrogenases).

本研究旨在探讨垂体前叶细胞间亲和力的调节机制。在腺体中，5种分泌细胞以有趣的方式分布。例如，LH/FSH细胞通常被催乳素细胞包围，而ACTH细胞经常位于GH细胞附近。此外，不产生激素的滤泡星状细胞具有相互粘附的倾向。基于这些观察结果，我们假设这些细胞类型之间通过细胞粘附进行信号转导，活细胞纯化和鉴定对于破译精确机制至关重要。最近，我们已经建立了两种不同的方法，利用凝集素和基因修饰的S100-b-GFP大鼠，这使我们能够纯化和识别活的激素产生细胞和活的卵泡星状细胞的垂体前叶细胞的混合人口，分别。从两种方法获得的数据表明，垂体前叶细胞中有三种局部调节机制：matricrine（基质的作用），acutacrine（细胞粘附分子的作用）和paracrine（局部表达的视黄醇脱氢酶）。

项目成果

Living-cell imaging of transgenic rat anterior pituitary sells in primary culture reveals novel characteristics of folliculo-stellate cells

原代培养中转基因大鼠垂体前叶的活细胞成像揭示了滤泡星状细胞的新特征

• 发表时间: 2010
• 期刊: J Endocrinol. 204
• 作者: Horiguchi;et al.
• 通讯作者: et al.

Estorogen receptor alpha regulates retinaldehyde dehyadrogenare 1 expression in trat anterior pituitary cells

雌激素受体α调节垂体前叶细胞视黄醛脱氢酶1的表达

• 发表时间: 2009
• 期刊: Endocr J. 56
• 作者: Fujiwara;et al.
• 通讯作者: et al.

ラット成体の腺下垂体にみられるE-カドヘリン陽性細胞の性質

成年大鼠腺垂体中E-钙粘蛋白阳性细胞的特征

• 发表时间: 2010
• 作者: Fujiwara K;Davaadash B;Kikuchi M;Takigami S;Yashiro T.;瀧上 周;藤原 研;藤原研;菊地元史;藤原研;藤原 研;菊地 元史;藤原 研;菊地元史
• 通讯作者: 菊地元史

Spatio-temporal relation between cadherin switching and cytogenesis of hormone-producing cell in the developing rat adenohypophysis.

发育中的大鼠腺垂体中钙粘蛋白转换与激素产生细胞的细胞发生之间的时空关系。

• 发表时间: 2009
• 期刊: Anat Sci Int. 84
• 作者: Kikuchi;et al.
• 通讯作者: et al.

In vivo correlation between c-Fos expression and corticotroph stimulation by adrenocorticotrophic hormone secretagogues in rat anterior pituitary gland

大鼠垂体前叶 c-Fos 表达与促肾上腺皮质激素促泌剂刺激促肾上腺皮质激素之间的体内相关性

• 发表时间: 2008
• 期刊: Cell and Tissue Research 331
• 作者: Horiguchi K;Fujiwara K;Kouki T;Kikuchi M;Yashiro T.;藤原研;瀧上周
• 通讯作者: 瀧上周