Protective mechanisms of water-soluble extract from culture medium of Ganoderma lucidum mycelia against neuronal damage after cerebral ischemia/reperfusion in diabetic state

灵芝菌丝体培养基水溶性提取物对糖尿病脑缺血/再灌注后神经元损伤的保护机制

• 批准号: 19590700
• 负责人: OKAZAKI Mari
• 金额: $ 3万
• 依托单位: Josai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590700/
• 关键词: 酸化ストレス; 糖尿病; 霊芝菌糸体培養培地抽出物(MAK); 一過性脳虚血; 炎症性サイトカイン; グルタミン酸トランスポーター; ストレプトゾトシン糖尿病; 中大脳動脈閉塞術(MCAO); 虚血再灌流障害; HMGB1; 霊芝菌糸体培養培地抽出物; 抗酸化食品; 炎症; 霊芝糸体培養地抽出物; 抗酸化酸素活性

Diabetic Mellitus (DM) have been shown to enhance oxidative stress leading to aggravation of cerebral ischemic injury following stroke. In this study, we examined the effects of oral administration of a water-soluble extract from culture medium of Ganoderma lucidum mycelia (MAK) on blood glucose level (BG), total plasma oxidative stress, and activity of antioxidant enzymes in DM animals. Furthermore, neuroprotective effects of MAK against exacerbation of neuronal damage induced by cerebral ischemia/reperfusion in the DM animals were investigated. DM animals had increased BG and decreased activity of antioxidant enzymes in the brain. DM animals treated with MAK had significantly lower BG and normal activity of the antioxidant enzyme. Treatment of MAK remarkably improved aggravated neurological deficits and cerebral injury induced by ischemia/reperfusion in diabetic animals. These results show that daily intake of MAK relieves the exacerbation of cerebral ischemic injury in a diabetic state, which may be attributed to improvement of augmented oxidative stress.

糖尿病（DM）已被证明可增强氧化应激，导致卒中后脑缺血性损伤加重。在这项研究中，我们研究了口服给药的水溶性提取物从培养基中的灵芝菌丝体（MAK）对血糖水平（BG），总血浆氧化应激和抗氧化酶的活性在DM动物的影响。此外，MAK对DM动物脑缺血/再灌注引起的神经元损伤的加重的神经保护作用进行了研究。DM动物的血糖增加，大脑中抗氧化酶的活性降低。用MAK治疗的DM动物具有显著较低的BG和正常的抗氧化酶活性。MAK治疗显著改善糖尿病动物缺血/再灌注引起的加重的神经功能缺损和脑损伤。这些结果表明，每日摄入MAK缓解了糖尿病状态下脑缺血性损伤的恶化，这可能归因于增强的氧化应激的改善。

项目成果

Hypoglycemic effects of a water-soluble extract from culture medium of Ganoderma lucidum (Rei-shi) mycelia in type 2 diabetic mice

灵芝菌丝体培养基水溶性提取物对 2 型糖尿病小鼠的降血糖作用

• 发表时间: 2010
• 期刊: Japanese Journal of Complementary and Alternative Medicine
• 作者: Shinya Kamiuchi;Yuko Hatta;Akane Miyazato;Mari Okazaki;Yukiko Kawahara;Aiko Tanaka;Yuri Shindou;Meiyan Xuan;Fumiko Suzuki;Hiroshi Iizuka and Yasuhide Hibino
• 通讯作者: Hiroshi Iizuka and Yasuhide Hibino

2型糖尿病マウスにおける霊芝菌糸体培養培地抽出物の血糖上昇抑制メカニズムの解析

灵芝菌丝体培养基提取物抑制2型糖尿病小鼠血糖升高的机制分析

• 发表时间: 2010
• 作者: 新藤由梨;その他
• 通讯作者: その他

糖尿病態ラットにおける中大脳動脈閉塞/再灌流処置後の脳障害と霊芝菌糸体培養培地抽出物(MAK)の脳保護効果

糖尿病大鼠大脑中动脉闭塞/再灌注治疗后脑损伤及灵芝菌丝体培养基提取物（MAK）的脑保护作用

• 发表时间: 2008
• 作者: 笠原知里;岡崎真理;岩田直洋;神内伸也;鈴木史子;飯塚博;日比野康英
• 通讯作者: 日比野康英

Inhibitory effects of a water-soluble extract from culture medium of Ganoderma lucidum (Rei-shi) mycelia on postprandial blood glucose elevation in mice and additional effect with α-glucosidase inhibitor.

灵芝菌丝体培养基的水溶性提取物对小鼠餐后血糖升高的抑制作用以及α-葡萄糖苷酶抑制剂的附加作用。

• 发表时间: 2007
• 期刊: Journal of Japanese Society of Nutrition and Food Science 60
• 作者: Usui;T.;Okazaki;M.;Kamiuchi;S.;Suzuki;F.;Iizuka;H. and Hibino;Y.
• 通讯作者: Y.

Antioxidant properties of a water-soluble extract from cluture medium of Ganoderma lucidum (Rei-shi) mycelia and antidiabetic effects in streptozotocin-treated mice.

灵芝（Rei-shi）菌丝体培养基中水溶性提取物的抗氧化特性以及链脲佐菌素治疗小鼠的抗糖尿病作用。

• 发表时间: 2008
• 期刊: Japanese Journal of Complementary and Alternative Medicine 5
• 作者: Okazaki M.;Tanaka A.;Hatta Y.;Kawahara Y.;Kamiuchi S.;Iwata N.;Asano S.;Suzuki F.;Iizuka H.;Hibino Y.
• 通讯作者: Hibino Y.