Development of the molecular imaging for proteins related to neuropathic pain

我们分析了囊泡GABA转运体异基因（VGAT+/-）小鼠，以了解GABA和甘氨酸的变化规律。小鼠似乎有较少的释放GABA和甘氨酸从突触前位点。我们研究了麻醉敏感性并检查感觉性能，比较了WT小鼠和VGAT+/-小鼠之间的急性伤害感受和炎性疼痛试验。此外，我们还检测了GABA能和甘氨酸能的微抑制性突触后电流（mIPSC）的电生理特性。在野生型（WT）小鼠和VGAT+/-小鼠之间比较了至麻醉作用的时间和麻醉作用的持续时间。为了测试脊髓上水平的感觉性能，我们测量了从52℃的热板上跳跃或舔的潜伏期。为了检查炎症疼痛信号，我们使用福尔马林测试。现在我们研究GABA能和甘氨酸能mIPSC，并比较两种基因型的两种电流的衰减和频率。

We analyzed vesicular GABA transporter heterogenic(VGAT+/-) mice to find out the rule of GABA and glycine. The mice are seemed to have less release of GABA and glycine from presynaptic sites. We studied anesthetic sensitiveness and to examine sensory performance, acute nociception and inflammatory pain tests were compared between WT mice and VGAT+/- mice. Also we examined GABAergic and glycinergic miniature inhibitory postsynaptic currents (mIPSC) by electrophysiological properties. The time to anesthetic effect nor the duration of anesthetic effect were compared between wild-type (WT) mice and VGAT+/- mice. To test the sensory performance at the supraspinal level, we measured the latency to jumping or licking from the hotplate set at 52℃. To examine about inflammatory pain signaling, we used the formalin test. Now we examine GABAergic and glycinergic mIPSC and compare abut decay and frequencies in two currents of both genotypes.