Changes and functions of pre-ovulatory human granulosa cells.

排卵前人颗粒细胞的变化和功能。

• 批准号: 19791136
• 负责人: KISHI Hiroshi
• 金额: $ 2.51万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19791136/
• 关键词: 生殖生理; シグナル伝達; 発現制御; 体外受精胚移植; 顆粒膜細胞; mRNA

With pre-ovulatory human granulosa cells, which are obtained on the occasion of ICSI therapy for infertile women and usually disposed, we analyzed the patterns of gene expression using quantitative PCR method.The amount of the cells obtained with this therapy is extremely small. Nevertheless, we succeeded in quantifying the expression of one gene. For other genes, we could not get enough result.

我们用ICSI治疗不孕妇女时获得的排卵前的人颗粒细胞，用定量PCR方法分析了基因表达的模式。然而，我们成功地量化了一个基因的表达。对于其他基因，我们不能得到足够的结果。