Control of nonlinear processes of protein solution and biological cells induced by focused femtosecond laser

聚焦飞秒激光引起的蛋白质溶液和生物细胞非线性过程的控制

• 批准号: 19686007
• 负责人: HOSOKAWA Youchiroh
• 金额: $ 16.47万
• 依托单位: Nara Institute of Science and Technology (2008-2010)Hamano Life Science Research Foundation (2007)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19686007/
• 关键词: フェムト秒レーザー; 原子間力顕微鏡; 蛋白質; 細胞; 衝撃波

When an intense infrared-femtosecond-laser is focused into biological materials, shockwave and cavitation bubble are generated at the laser focal point, in which an explosive morphological change of water is induced by an effective multiphoton absorption. I developed a new local force measurement system to estimate the impulsive force due to the shockwave and cavitation bubble generated in biological materials and elucidated time and spatial evolution of these phenomena. On the basis of this knowledge, new control and measurement methods of single cell or intracellular protein were devised.

当强红外飞秒激光聚焦到生物材料中时，激光焦点处会产生冲击波和空化气泡，有效的多光子吸收会导致水的爆炸性形态变化。我开发了一种新的局域力测量系统来估计生物材料中产生的冲击波和空化气泡产生的冲击力，并阐明了这些现象的时间和空间演化。在此基础上，设计了新的单细胞或胞内蛋白的控制和测量方法。

项目成果

Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy

• DOI: 10.1073/pnas.1006847108
• 发表时间: 2011-02-01
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Hosokawa, Yoichiroh;Hagiyama, Man;Ito, Akihiko
• 通讯作者: Ito, Akihiko

Induction of the cell-cell connections by using in-situ laser lithography on a perfluoroalkyl-layered cultivation platform.

在全氟烷基分层培养平台上使用原位激光光刻诱导细胞间连接。

• 发表时间: 2011
• 期刊: Chem Bio Chem Vol.12
• 作者: K.Okano;D.Yu;A.Matsui;Y.Maezawa;Y.Hosokawa;A.Kira;M.Matsubara;I.Liau;H.Tsubokawa;H.Masuhara.
• 通讯作者: H.Masuhara.

フェムト秒レーザーによる個別細胞パターニングプロセス

使用飞秒激光的单个细胞图案化工艺

• 发表时间: 2007
• 作者: 細川 陽一郎;宇和田 貴之;瀧澤 典子;岡野 和宣;増原 宏
• 通讯作者: 増原 宏

Nanoparticles injection into single animal cells by femtosecond laser-induced impulsive force

通过飞秒激光诱导脉冲力将纳米粒子注射到单个动物细胞中

• 发表时间: 2007
• 作者: A.Yamaguchi;Y.Hosokawa;G.Louit;T.Asahi;H.Masuhara;C.Shukunami;Y.Hiraki.
• 通讯作者: Y.Hiraki.

Cell Viability after Femtosecond Laser Ablation of Tissue

飞秒激光组织消融后的细胞活力

• 发表时间: 2007
• 作者: Yoichiroh Hosokawa;Noriko Takizawa;Takayuki Uwada;Kazunori Okano;Hiroshi Masuhara
• 通讯作者: Hiroshi Masuhara