Cellular and molecular responses to elevated pressure stimulus in skeletal myocytes

骨骼肌细胞对高压刺激的细胞和分子反应

• 批准号: 19700525
• 负责人: MORITA Noriteru
• 金额: $ 2.52万
• 依托单位: Hokkaido University of Education (2008-2009)Asai Gakuen University (2007)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19700525/
• 关键词: メカニカルストレス; 筋細胞; myogenesis; 圧力

It is not yet clear that how mechanical stimuli during muscle contraction, such as stretch and pressure stresses, affect skeletal myocyte differentiation. The purpose of this study was to examine the effects of pure pressure stimulus on muscle differentiation in differentiating myoblasts. Atmospheric pressure at 160 mmHg, which is equivalent to calf muscle intramuscular pressure during locomotion, was applied to early phase of L6 myoblast differentiation. Compared to controls kept under normal pressure, the number of fused myoblasts reduced after pressurization. Myogenin and myosin heavy chain proteins in the pressurized cells were lowered. These results suggest that elevated intramuscular pressure may suppress myogenic differentiation from myoblasts to myotubes in contracted skeletal muscles.

目前还不清楚肌肉收缩过程中的机械刺激，如拉伸和压力应激，如何影响骨骼肌细胞的分化。本研究的目的是研究纯压力刺激对成肌细胞分化过程中肌肉分化的影响。在L6成肌细胞分化的早期阶段，施加相当于运动过程中小腿肌肉内压力的160 mm Hg大气压。与常压对照组相比，加压后融合成肌细胞的数量减少。加压后细胞内的肌球蛋白和肌球蛋白重链蛋白降低。这些结果表明，在收缩的骨骼肌中，肌内压力的升高可能会抑制成肌细胞向肌管的分化。

项目成果

Optimizing a Protocol of Low Intensity Resistance Training with Blood Flow Restriction

优化血流限制低强度阻力训练方案

• 发表时间: 2008
• 作者: T Suga;K Okita;N Morita;M Horiuchi;T Yokota;K Hirabayashi;S Kinugawa;H Tsutsui
• 通讯作者: H Tsutsui

Exposure to Pressure Stimulus Suppresses Myogenin Expression in Differentiating L6 Myoblasts.

暴露于压力刺激会抑制分化 L6 成肌细胞中的肌生成素表达。

• 发表时间: 2009
• 作者: Noriteru Morita;Kenji Iizuka;Yoshiki Fujisawa;Takuji Machida;Masahiro Horiuchi;Masahiko Hirafuji;Koichi Okita
• 通讯作者: Koichi Okita

Mechanical pressure activates the glucose metabolism and MAPK signal ing in L6 skeletal myocytes

机械压力激活 L6 骨骼肌细胞中的葡萄糖代谢和 MAPK 信号传导

• 发表时间: 2008
• 作者: 藤沢佳輝;飯塚健治;森田憲輝;町田拓自;平藤雅彦;N. Morita
• 通讯作者: N. Morita

筋芽細胞への圧力刺激はmyogenin発現を抑制する

成肌细胞的压力刺激抑制肌细胞生成素表达

• 发表时间: 2008
• 作者: 森田憲輝;飯塚健治;藤沢佳輝;町田拓自;平藤雅彦;沖田孝一
• 通讯作者: 沖田孝一

Effects of pressure stimulus on cell proliferation and differentiation in L6 skeletal muscle cells

压力刺激对L6骨骼肌细胞增殖和分化的影响

• 发表时间: 2008
• 作者: 藤沢佳輝;飯塚健治;森田憲輝;町田拓自;平藤雅彦
• 通讯作者: 平藤雅彦