Cellular and molecular responses to elevated pressure stimulus in skeletal myocytes

目前还不清楚肌肉收缩过程中的机械刺激，如拉伸和压力应激，如何影响骨骼肌细胞的分化。本研究的目的是研究纯压力刺激对成肌细胞分化过程中肌肉分化的影响。在L6成肌细胞分化的早期阶段，施加相当于运动过程中小腿肌肉内压力的160 mm Hg大气压。与常压对照组相比，加压后融合成肌细胞的数量减少。加压后细胞内的肌球蛋白和肌球蛋白重链蛋白降低。这些结果表明，在收缩的骨骼肌中，肌内压力的升高可能会抑制成肌细胞向肌管的分化。

It is not yet clear that how mechanical stimuli during muscle contraction, such as stretch and pressure stresses, affect skeletal myocyte differentiation. The purpose of this study was to examine the effects of pure pressure stimulus on muscle differentiation in differentiating myoblasts. Atmospheric pressure at 160 mmHg, which is equivalent to calf muscle intramuscular pressure during locomotion, was applied to early phase of L6 myoblast differentiation. Compared to controls kept under normal pressure, the number of fused myoblasts reduced after pressurization. Myogenin and myosin heavy chain proteins in the pressurized cells were lowered. These results suggest that elevated intramuscular pressure may suppress myogenic differentiation from myoblasts to myotubes in contracted skeletal muscles.