Identification of novel AKT regulating molecules.

在酵母双杂交实验中，我们发现TTC3位于唐氏综合征关键区21号染色体上，特异地与Akt相互作用。TTC3优先与磷酸化的Akt结合，并主要在细胞核内促进其泛素化/降解。通过共聚焦显微镜观察，TTC3在整个细胞周期中与Akt共定位，表明TTC3通过控制Akt在细胞核内的活性来调节细胞存活和增殖。（Suizu等人）发育细胞（2009）

In yeast two-hybrid assays, we found that TTC3, which is located at chromosome 21 within the Down Syndrome Critical Region, specifically interacted with Akt. TTC3 preferentially binds to phosphorylated Akt and facilitates its ubiquitination/degradation predominantly within the nucleus. By confocal microscopy the TTC3 co-localizes with Akt throughout the cell cycle, suggesting that TTC3 regulate cell survival and proliferation by controlling Akt activity within the nucleus. (Suizu et al. Developmental Cell 2009)