Effects of protein/protein interaction on activity of drug metabolizing enzymes

细胞色素 P450 (CYP) 是最重要的药物代谢酶之一。在这项研究中，我们研究了蛋白质/蛋白质相互作用对 CYP 酶活性的影响。哺乳动物双杂交系统和免疫沉淀测定表明，在用 myc 或 flag 标签蛋白表达质粒转染的细胞中，CYP3A4 与 CYP3A4 或 CYP3A5 相互作用。 CYP3A4 还与 CYP2C 亚型相互作用。这些结果表明 CYP 在细胞中相互作用，并且这些相互作用可能与 CYP 表达或功能相关。

Cytochrome P450 (CYP) is one of the most important drug-metabolizing enzymes. In this study, we investigated the effects of protein/protein interaction on the enzyme activity of CYPs. Mammalian two-hybrid system and immunoprecipitation assay revealed that CYP3A4 interacted with CYP3A4 or CYP3A5 in the cells transefected with expression plasmids for myc- or flag-tagged proteins. CYP3A4 also interacted with CYP2C isoforms. These results suggest the CYPs interact with each other in the cells, and possibly these interactions were associated with CYP expression or function.