Characteristics of Oral Tissue-Derived MSCs from CLP Patients

尽管越来越多的报道使用间充质干细胞的牙槽腭骨重建在CLP患者，目前尚不清楚是否从CLP患者的口腔组织来源的间充质干细胞能够分化成骨细胞谱系和再生新骨相媲美，其匹配的对照。本研究旨在检测唇腭裂患者DPSCs的特性。更具体地说，该研究分析了与健康匹配对照相比，来自CLP患者的这些细胞的自我更新活性和多谱系分化潜力。对16例患者的24颗离体牙进行分析。11颗牙齿（CLP患者）和13颗牙齿（对照健康患者）。牙齿在正畸治疗前需要拔除，但因恒牙而拔除的乳牙除外。CLP患者和对照组的DPCSs和PDL细胞增殖无差异。CLP组DP细胞与PDL细胞比较，第1天PDL细胞的增殖率明显高于DP细胞（P<0.05）。CLP组细胞的PDT与健康对照组相当。CFU测定观察到类似的观察结果。茜素红染色、碱性磷酸酶（ALP）、Runx 2和骨桥蛋白（OPN）mRNA表达检测DPSCs向成骨细胞分化的能力。CLP组和对照组细胞在成骨培养液中均有钙沉积。在基础培养基中培养的细胞中未观察到染色。以MC 3 T3-E1成骨样细胞为阳性对照。

Despite increasing reports on the use of MSCs for alveolar palate bone reconstruction in CLP patients, it remain unclear whether MSCs of oral tissue origin taken from CLP patients are capable to differentiate into osteoblastic lineage and regenerate new bone comparable as to its matched controls. This study aims to examine the characteristics of DPSCs taken from cleft lip palate patients. More specifically, the study analyze the self-renewal activity and multi-lineage differentiation potential of these cells from CLP patients in comparison to healthy matched controls. Analyses were carried out from 24 extracted tooth were collected from 16 patients. 11 teeth (CLP patients) and 13 teeth (control healthy patients). The teeth were indicated for extraction prior to orthodontic treatment, except for deciduous teeth that were extracted due to persistence condition. No differences in the cell proliferation of DPCSs and PDL Cells between CLP patients and control. However comparison of DP cells and PDL cells in CLP group showed higher cells proliferation at Day 1 in PDL cells compared to DP Cells (P<0.05). PDT of cells from CLP group were comparable with control healthy group. Similar observation was seen for CFU assay. The ability of DPSCs to differente into osteoblastic lineage was examined by Alizarin red staining, ALP, Runx2 and OPN mRNA expression. Calcium deposition were detected in cells from CLP and Control group cultured in osteogenic medium. No staining was seen in the cells cultured in basal medium. MC3T3-E1 osteoblast-like cells were used as positive control.