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Pathohistological diagnosis of infiltration and metastasis of tongue carcinoma : Three-dimensional analysis of tumor cell atypia and alveolar architecture

舌癌浸润转移的病理组织学诊断：肿瘤细胞异型性和牙槽结构的三维分析

基本信息

批准号：
19592321
负责人：
YAGISHITA Hisao
金额：
$ 2.83万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

We initiated histology-based 3D reconstruction of oral squamous cell carcinoma (SCC) arising on the lateral border of the tongue in order to collect quantitative information of diagnostic value regarding cancer invasion and prognosis. The basic procedures for 3D reconstruction are: preparation of serial histological sections in combination with immunostaining of cell/tissue constituents of interest, alignment and superposition of digitized images, and computer-assisted color segmentation of labeled targets. Cytoplasm/nucleus segmentation of individual SCC cells lead to quantitative analysis of histological parameters. The results support the wide potential usage and advantage of histology-based 3D reconstruction in cancer biology understanding and pathological diagnosis. Particularly, 3D histomorphometric parameters regarding tumor alveolar structure and spatial distribution of cancer foci in a microenvironment will be of inexpensive value in prediction and diagnosis of tumor aggressiveness and malignancy.

我们对舌侧缘发生的口腔鳞状细胞癌（SCC）进行了基于组织学的三维重建，以收集对肿瘤侵袭和预后有诊断价值的定量信息。三维重建的基本步骤是：结合感兴趣的细胞/组织成分的免疫染色制备连续组织学切片，数字化图像的对齐和叠加，以及标记目标的计算机辅助颜色分割。单个SCC细胞的细胞质/细胞核分割导致组织学参数的定量分析。结果支持基于组织学的三维重建在癌症生物学理解和病理诊断中的广泛潜在应用和优势。特别是，关于肿瘤肺泡结构和肿瘤病灶在微环境中的空间分布的三维组织形态学参数在预测和诊断肿瘤侵袭性和恶性方面将具有廉价的价值。