A liposome encapsulating molecular computer : an autonomously judging drug delivery system.

我开发了一种脂质体封装分子计算机：RTRACS。本研究成功地合成了一种能使DNA/RNA分子穿过脂质双层膜的分子伞（DNA-胆酸偶联物）。为了检查脂质体的局限性，将一个巨大的脂质体应用于PCR条件（高温和强对流）。因此，在PCR条件下，巨脂质体保持了形状和屏障能力。该单层脂质体可用于脂质体DDS的研究。因此，我们在经典的Bangham法（脂膜自然溶胀法）的基础上发展了一种制备巨单层囊泡的新方法。许多GUV可以在生理条件下（含有150 mM NaCl和10 mM MgCl 2）在没有油的支持下制备。

I had developed a liposome encapsulating molecular computer : RTRACS. In the present study, a molecular umbrella (DNA-cholic acid conjugate) which permeated DNA/RNA molecule through lipid bilayer membrane was successfully synthesized. In order to check the limitation of liposome, a giant liposome was applied to PCR conditions (high temperature and hard convection). Consequently the giant liposome maintained the shape and barrier capability under the PCR conditions. The unilamellar liposome is useful for the liposome-based DDS research. Therefore we developed a novel method for giant unilamellar vesicle (GUV) preparation which based on the classical Bangham's method (natural swelling method of lipid film). Many GUVs can be prepared in the physiological condition (containing 150mM NaCl and 10mM MgCl2) without the support of oil.