Identification of neurons by fluorescence spectrogram through a patch electrode, and the application to auditory neural circuits in vivo

本研究旨在记录脑深部神经元电活动的同时记录光信号。为此，我们开发了一种利用贴片电极作为光导的光度学贴片电极(PME)记录系统。用俄勒冈绿BAPTA-1-AM(OGB-1)填充雏鸡听觉核团神经元，在鸡的L野、鸟类听皮质和下丘记录到细胞内钙信号与声刺激反应的电信号。阻断L区GABA受体后，慢场电流增强，增强了与电慢波同步产生的钙波。在下丘，记录到类似的钙反应，阻断GABA受体后，钙反应增强。因此，我们成功地开发了PME记录系统，通过贴片电极与光学信号同步监测脑深部组织中的神经元电活动。

This study aimed at recording optical signal simultaneously with neuronal electrical activity in the deep brain tissue. For that purpose, we developed a photometric patch electrode (PME) recording system by using a patch electrode as a light guide. By filling the neuron in the auditory nuclei of a chick with Oregon green BAPTA-1-AM (OGB-1), intracellular Ca2+signals were recorded simultaneously with electrical signal in response to acoustic stimulus in the Field-L, the avian auditory cortex, and in the inferior colliculus. A slow field current was enhanced after the block of GABA receptor in the Field-L, which enhanced the Ca2+wave that was generated in synchrony with the electrical slow wave. In the inferior colliculus, Ca2+responses were similarly recorded and were enhanced after the block of GABA receptor. Therefore, we succeeded in development of PME recording system to monitor neuronal electrical activity simultaneously with optical signal through the patch electrode in the deep brain tissue.