Epigenome analysis of cancer

本研究的目的是明确去甲基化剂对骨髓增生异常综合征（MDS）患者的影响。到目前为止，我们已经从4名MDS患者（2名患有RCMD，2名患有RAEB-2）以及从两名健康供体（商业产品）构建了启动子甲基化组。来自两种不同亚型的样本聚集在一起，DNA甲基化分析似乎可以作为一种有用的诊断方法。我们发现了一个巨大的过度表达的转录因子内确定的基因组之间的差异甲基化的两种肿瘤类型。大多数其他基因也可以被认为具有调节功能，其中许多基因编码参与信号转导的蛋白质，以及许多microRNA基因。我们还研究了与这些基因组相关的生物过程。对于大多数分析，我们发现与各种发育过程相关的基因的过度表达，其中许多基因涉及神经系统发育。

The purpose of this study is to specify the effect of de-methylating agents on myelodysplastic syndrome (MDS) patients. So far, we have constructed promoter methylomes from 4 MDS patients (2 with RCMD and 2 with RAEB-2), as well as that from two healthy donors (commercial products). Samples from the two different subtypes clustered together, and it seems likely that DNA methylation analysis could serve as a useful diagnostic. We found an enormous over-representation of transcription factors within sets of identified genes differentially methylated between the two tumor types. Most other genes identified could also be considered to have regulatory functions with many genes encoding proteins involved in signal transduction, as well as a number of microRNA genes. We also looked at biological process associated with these gene sets. For most analyses we found an even more enormous over-representation of genes associated with various developmental processes, with many genes involved in nervous system development.