Generation of a New Class of Catalytic Antibodies based Directed Evolution

在这项研究中，我们尝试通过定向进化和新设计的半抗原免疫两种方式来产生一类新的催化抗体。我们开发了一个酵母表面显示的催化抗体文库，显示出类似荧光素酶的活性。此外，我们证明了一种单一抗体通过产生抗原结合位点催化多种化学转化，该抗原结合位点作为载脂蛋白结合功能化的小非蛋白质成分。用酸性和氨基辅因子替代该辅因子使抗体能够以较大的速率加速催化β-消除、脱羧和醛醇反应。

In this study we have tried to generate a new class of catalytic antibodies by two ways, directed evolution and newly designed hapten immunization. We have developed a yeast surface-displayed library of catalytic antibodies showing a luciferase-like activity. In addition, we demonstrate a single antibody catalyzing multiple chemical transformations by the generation of antigen-combining site that function as an apoprotein for binding functionalized small non-protein components. Replacement of this cofactor with acidic and amino cofactors enabled the antibodies to catalyze β-elimination, decarboxylation and aldol reactions with large rate accelerations.