The calcium signaling pathway that initiates embryonic development in fertilize amphibian eggs.

启动两栖动物受精卵胚胎发育的钙信号通路。

• 批准号: 20370085
• 负责人: OHSUMI Keita
• 金额: $ 13.23万
• 依托单位: Nagoya University (2009-2010)Tokyo Institute of Technology (2008)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20370085/
• 关键词: 胚発生; 受精; カルシウムシグナル; カエル卵

At fertilization, a transient increase of cytoplasmic calcium ions(Ca^<2+>) triggers various activation responses in animal eggs. In amphibian, these responses include exit from meiotic arrest and cortical remodeling observed as activation contraction. We have investigated substrates for calcineurin in inducing the meiotic exit and regulatory mechanisms for the activation contraction, using cell-free extracts and cell cortices isolated from Xenopus eggs. The results indicated that Erp1 and Cdc20, both of which are essential for cyclin B destruction which leads to the meiotic exit, are dephosphorylated upon activation. The phosphatase(s) responsible for their dephosphorylation remains to be identified. We have also established the method to isolate the cell cortex from oocytes and eggs and to elute their cortical proteins. Using these materials, we found that anillin, an actin cytoskeletal regulator protein, increases during oocytes maturation and is dephosphorylated upon activation. Thus, the cortical isolation method developed in this study has enabled the biochemical analyses of cell cortex.

在受精时，细胞质钙离子（Ca^2+）的瞬时增加触发动物卵子中的各种激活反应。在两栖动物中，这些反应包括退出减数分裂停滞和皮质重塑观察到的激活收缩。我们研究了底物钙调神经磷酸酶诱导减数分裂退出和调节机制的激活收缩，使用无细胞提取物和细胞皮层分离爪蟾卵。结果表明，Erp 1和Cdc 20，这两个都是必不可少的细胞周期蛋白B的破坏，导致减数分裂退出，被激活后去磷酸化。负责其去磷酸化的磷酸酶仍有待鉴定。我们还建立了从卵母细胞和卵子中分离细胞皮层和纯化其皮层蛋白的方法。使用这些材料，我们发现，苯胺，肌动蛋白细胞骨架调节蛋白，增加在卵母细胞成熟和激活后去磷酸化。因此，在这项研究中开发的皮层分离方法，使细胞皮层的生化分析。

项目成果

Meiosis-II arrest and its cancellation in Xenopus eggs

爪蟾卵减数分裂 II 的停滞及其取消

• 发表时间: 2008
• 作者: K. Ohsumi et;al.
• 通讯作者: al.

核内膜タンパク質LBRによる初期胚の核の構造と機能の制御

内核膜蛋白LBR对早期胚胎核结构和功能的调节

• 发表时间: 2009
• 作者: 岩渕万里;大隅圭太
• 通讯作者: 大隅圭太

脊椎動物の未受精卵における減数分裂停止とその解除

未受精脊椎动物卵减数分裂的停滞和释放

• 发表时间: 2008
• 期刊: 蛋白質 核酸 酵素 53
• 作者: 地道 拓志;藤田 英明;赤木 泰文;西山 朋子
• 通讯作者: 西山 朋子

Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vitro

体外体细胞转分化需要卵母细胞型接头组蛋白 B4

• 发表时间: 2010
• 期刊: FASEB Journal
• 影响因子: 4.8
• 作者: Maki;N;et al
• 通讯作者: et al

Meiosis arrest and its cancellation in Xenopus eggs

非洲爪蟾卵减数分裂的停滞和取消

• 发表时间: 2008
• 作者: Ohsumi;K.
• 通讯作者: K.