Development of DNA markers and QTL mapping relating to oleic acid contents in peanut.

花生油酸含量相关DNA标记及QTL定位的开发

• 批准号: 20380008
• 负责人: TABATA Satoshi
• 金额: $ 12.06万
• 依托单位: Kazusa DNA Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380008/
• 关键词: 植物; 育種; ゲノム; 遺伝学; 脂質; 落花生; 脂肪酸; DNAマーカー; オレイン酸; 連鎖地図; SNP; FAD遺伝子

In order to accelerate breeding for high oleic acid contents in peanut, two selection markers were developed from candidate gene sequences controlling oleic acid contents. In addition, genome wide polymorphic DNA markers (microsatellite and transposon element markers) were developed and mapped on genetic linkage maps of peanut. Those genome wide DNA markers as well as the two candidate gene markers were used for marker assisted selection of the high-oleic peanut breeding program. As a result, we succeed to reduce breeding periods from five to three years.

为了加快花生高油酸育种的步伐，利用控制油酸含量的候选基因序列开发了两个选择标记。此外，还开发了全基因组多态性DNA标记（微卫星标记和转座子标记），并将其定位在花生遗传连锁图谱上。利用这些标记和这两个候选基因标记对高油酸花生进行分子标记辅助选择。因此，我们成功地将繁殖期从五年缩短到三年。

项目成果

Large-scale development of EST-derived SSR markers and diersity analysis in Arachis spp.

花生 EST 衍生 SSR 标记的大规模开发和多样性分析。

• 期刊: Molecular Breeding
• 影响因子: 3.1
• 作者: Padmalatha Koilkonda;Shusei Sato;Satoshi Tabata;Kenta Shirasawa;Hideki Hirakawa;Hiroe Sakai;Shigemi Sasamoto;Akiko Watanabe;Tsuyuko Wada;Yoshie Kishida;Hisano Tsuruoka;Tsunakazu Fujishiro;Manabu Yamada;Mitsuyo Kohara;Shigeru Suzuki;Makoto H
• 通讯作者: Makoto H

Development of microsatellite, single nucleotide polymorphism, and transposon markers for peanut breeding.

花生育种微卫星、单核苷酸多态性和转座子标记的开发。

• 发表时间: 2009
• 作者: Shirasawa K;Koilkonda P;Naito Y;Watanabe M;Suzuki S;Hasegawa H;Kiyoshima H;Kuwata C;Tabata S;Isobe
• 通讯作者: Isobe

Getting across the Devil river in the Legume land

在豆科之地渡过魔鬼河

• 发表时间: 2010
• 作者: Sachiko Isobe;Kenta Shirasawa;Shusei Sato;Hideki Hirakawa;Padmalatha Koilkonda;Satoshi Tabata
• 通讯作者: Satoshi Tabata

Marker assisted backcrossing selection(MABS)for oleic acid content in peanut

花生油酸含量的标记辅助回交选择（MABS）

• 发表时间: 2009
• 作者: Shirasawa;Koilkonda;Isobe;ら
• 通讯作者: ら

Development of microsatellite, SNP and AFLP markers in cultivated peanut

栽培花生微卫星、SNP 和 AFLP 标记的开发

• 发表时间: 2008
• 作者: Isobe S;Sato S;Shirasawa K;Suzuki S;Watanabe M;Tabata S
• 通讯作者: Tabata S