Analysis of key factor in fruit ripening using DNA array, ripening-impaired mutant and transgenic tomato

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We screened ripening key genes using tomato DNA macroarray consisted of 12, 000 clones, ripening-impaired mutants and transgenic technology. Ripening-regulated 428 genes were isolated and 244 genes were regulated under ethylene signaling. As ripening-regulated novel transcription factor or signaling factor, GRAS, SAHH, b-Zip and BTB genes were identified, suggesting their involvement in regulation of fruit ripening at the downstream of ethylene signaling. Transgenic tomatoes with all known LeEILs suppressed, exhibited non-ripening phenotype and indicated that onset of ripening-ethylene is regulated by developmental factors. Based on information in tomato, low-temperature induced ripening in kiwi fruit was analyzed and it was shown to be independent of ethylene signaling.