Molecular analysis of hormonal orchestration that induces various seasonal polyphenisms

为了分析Orgyia the llina的生殖多型性，包括滞育诱导的分子机制，我们首次克隆并鉴定了滞育信息素生物合成激活神经肽（DH-PBAN）cDNA，该cDNA编码5种Phe-X-Pro-Arg-Leu-NH_2（FXPRLa）神经肽。我们发现，Orgyia DH不仅诱导胚胎滞育，而且这种神经肽诱导季节性多型，参与卵泡和卵巢的肥大。这是第一个研究表明，神经肽具有多效性的影响，在季节性生殖多型，以实现季节性适应。我们还表明，一个新的因素（i。例如，DH神经肽）作为一种重要的季节性多表型诱导物，其是生活史权衡的基础。

To analyze the molecular mechanisms involved in reproductive polyphenism, including the diapause induction of Orgyia the llina, we first cloned and characterized the diapause hormone-pheromone biosynthesis activating neuropeptide(DH-PBAN) cDNA encoding the five Phe-X-Pro-Arg-Leu-NH_2(FXPRLa) neuropeptides. We revealed that not only does the Orgyia DH induce embryonic diapause, but also that this neuropeptide induces seasonal polyphenism, participating in the hypertrophy of follicles and ovaries. This is the first study showing that a neuropeptide has a pleiotropic effect in seasonal reproductive polyphenism to accomplish seasonal adaptation. We also show that a novel factor(i. e., the DH neuropeptide) acts as an important inducer of seasonal polyphenism underlying a life-history tradeoff.