Systematic screening of viral host factors using a transposon-tagging method.

我们克隆了青鳉HNI-2细胞系，其染色体数目为25。然后，我们将转座子引入细胞中进行基因破坏，并获得了17个抗β-odavirus和GFP表达的细胞系（GFP表达表明转座子插入）。从由每个病毒抗性细胞制备的基因组DNA中分离与插入的转座子相邻的DNA片段，并用于针对Ensembl青鳉基因组数据库的blaerobic搜索。从17个耐药细胞系中鉴定出的9个序列与数据库序列相符，其中5个序列被发现编码蛋白质。

We cloned a medaka (Oryzias latipes) HNI-2 cell line, the chromosome number of which was 25. We then introduced a transposon into the cells for gene disruption and obtained 17 betanodavirus-resistant and GFP-expressed cell lines (GFP expression indicates transposon insertion). DNA fragments adjacent to the inserted transposon were isolated from the genomic DNA prepared from each of the virus-resistant cells and used for blastn searches against the Ensembl medaka genome database. Nine sequences identified from the 17 resistant cell lines hit database sequences and five of which were revealed to encode proteins.