Systematic screening of viral host factors using a transposon-tagging method.

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We cloned a medaka (Oryzias latipes) HNI-2 cell line, the chromosome number of which was 25. We then introduced a transposon into the cells for gene disruption and obtained 17 betanodavirus-resistant and GFP-expressed cell lines (GFP expression indicates transposon insertion). DNA fragments adjacent to the inserted transposon were isolated from the genomic DNA prepared from each of the virus-resistant cells and used for blastn searches against the Ensembl medaka genome database. Nine sequences identified from the 17 resistant cell lines hit database sequences and five of which were revealed to encode proteins.