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The development of new control technology in CO2 and N2O emission by symbiotic microorganisms.

共生微生物CO2和N2O排放控制新技术开发。

基本信息

批准号：
20380176
负责人：
NARISAWA Kazuhiko
金额：
$ 12.31万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380176/
关键词：
環境修復根部エンドファイト共生生態

项目摘要

We selected a key species of the dark septate endophytic fungus (DSE), Phialocephala fortinii, which promotes the growth of host plant even under the stressed conditions. We showed that the appropriate growth conditions in nursery set including suitable nutrient and carbon concentrations need to be developed to ensure the formation of the symbiotic association between the DSE fungi and the host plants. The improvement in the colonization rate by P. fortinii was confirmed by adding the amino acid.Two strains of Veronaeospsis simplex, Y34 and IBA K45, which belong to the DSE have been selected. Interestingly, the presence of bacterial communities living outside and inside mycelium of both strains was revealed by fluorescence microscopic observation and confirmed further by the detection of endotoxin in mycelium as well. We attempted to control these associated bacteria using continuous antibiotic treatment and also using physical removal method to clarify the role of these associated bacteria. Terminal-restriction fragment length polymorphism (T-RFLP) coupled with the sequence data from 16S rDNA gene cloning detected the genera Pseudomonas and Stenotrophomonas in the Proteobacteria on both fungal wild strains, while the genus Microcystis in the Cyanobacteria and Delftia in the Proteobacteria were only detected in wild strain of IBA K45 and Y34, respectively.

我们选择了一个关键种的暗隔内生真菌，Phialocephala fortinii，它促进宿主植物的生长，即使在胁迫条件下。我们发现，在苗圃设置，包括合适的营养和碳浓度需要开发，以确保DSE真菌和宿主植物之间的共生关系的形成。通过添加氨基酸，证实了福氏平革菌的定殖率得到了提高。有趣的是，通过荧光显微镜观察揭示了两种菌株菌丝体内外存在细菌群落，并通过检测菌丝体中的内毒素进一步证实了这一点。我们试图通过持续的抗生素治疗来控制这些相关细菌，并使用物理去除方法来阐明这些相关细菌的作用。末端限制性片段长度多态性（T-RFLP）结合16 S rDNA基因克隆的序列数据检测到两种真菌野生菌株上的变形菌门的假单胞菌属和寡养单胞菌属，而蓝藻门的微囊藻属和变形菌门的Delftia属仅分别在IBA K45和Y34野生菌株中检测到。