The development of new control technology in CO2 and N2O emission by symbiotic microorganisms.

我们选择了一种即使在逆境条件下也能促进寄主植物生长的暗隔内生真菌phalocephala fortini。研究表明，为了确保DSE真菌与寄主植物之间形成共生关系，需要在苗圃设置适当的生长条件，包括适当的营养和碳浓度。通过添加氨基酸证实了福氏假单胞菌定殖率的提高。选取了属于DSE的2株单纯维罗纳绦虫Y34和IBA K45。有趣的是，通过荧光显微镜观察发现两株菌株菌丝内外均存在细菌群落，并通过菌丝内毒素检测进一步证实。我们试图通过持续的抗生素治疗来控制这些相关细菌，并使用物理去除方法来阐明这些相关细菌的作用。末端限制性片段长度多态性（T-RFLP）结合16S rDNA基因克隆的序列数据，在两个真菌野生菌株中均检测到变形菌门中的假单胞菌属和窄养单胞菌属，而在变形菌门中的微囊藻属和Delftia属分别仅在IBA K45和Y34野生菌株中检测到。

We selected a key species of the dark septate endophytic fungus (DSE), Phialocephala fortinii, which promotes the growth of host plant even under the stressed conditions. We showed that the appropriate growth conditions in nursery set including suitable nutrient and carbon concentrations need to be developed to ensure the formation of the symbiotic association between the DSE fungi and the host plants. The improvement in the colonization rate by P. fortinii was confirmed by adding the amino acid.Two strains of Veronaeospsis simplex, Y34 and IBA K45, which belong to the DSE have been selected. Interestingly, the presence of bacterial communities living outside and inside mycelium of both strains was revealed by fluorescence microscopic observation and confirmed further by the detection of endotoxin in mycelium as well. We attempted to control these associated bacteria using continuous antibiotic treatment and also using physical removal method to clarify the role of these associated bacteria. Terminal-restriction fragment length polymorphism (T-RFLP) coupled with the sequence data from 16S rDNA gene cloning detected the genera Pseudomonas and Stenotrophomonas in the Proteobacteria on both fungal wild strains, while the genus Microcystis in the Cyanobacteria and Delftia in the Proteobacteria were only detected in wild strain of IBA K45 and Y34, respectively.