Molecular machinery of granule exocytosis in pancreatic α and β cells

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We have analyzed the functional significance of lipid and protein interactions involving the Rab27a effector, granuphilin, in both cultured cells and genetically engineered mice. We have also found that another Rab27a effector, exophilin8, transiently traps insulin granules into the cortical actin network close to the microtubule plus-ends and supplies them for release during the stimulation. Finally, using a proteomics approach, we have systemically identified Rab27a-interacting molecules in both α and β cells.