Proteomic analysis of constriction mechanisms in response to oxygen in the ductus arteriosus

检测到氧敏感电压门控钾通道Kv1.5与Kvb1.2、α肌动蛋白-2、SAP97、含有Valosin的蛋白、网织蛋白、热休克蛋白60、三磷酸腺苷合成酶F1α/β亚基、α-肌动蛋白、禁止蛋白-1/-2直接或间接相互作用。这些蛋白可能在Kv1.5的细胞转运和调控中发挥作用。与动脉导管(DA)和肺动脉(PA)肌浆网(SR)内钙储存相关的基因表达随血管和胚胎发育阶段的不同而变化。根据发育阶段的不同，多巴胺中的SR活性可能具有特异性。

Direct or indirect interaction of oxygen-sensing voltage-gated potassium channel 1.5 (Kv1.5) with Kvb1.2, α actinin-2, SAP97, valosin containing protein, clathrin, HSP60, ATP synthase F1 α/βsubunits, α-actinin, prohibitin-1/-2was detected. These proteins might have roles in the cellular transportation and regulation of Kv1.5. Gene expression related to the storage of Ca2+in the sarcoplasmic reticulum (SR) in ductus arteriosus (DA) and pulmonary artery (PA) varied depending on the vessels and fetal developmental stages. The SR activity in the DA might be specific depending on the developmental stage.