The establishment of Pacemaker cell line from human amnion delivered cells, and development of the new pacing therapy

为了将羊膜细胞作为替代机械起搏器植入的新疗法进行起搏器细胞替代治疗，我们开发了从人羊膜细胞中纯化Nanog阳性细胞，并将Oct4基因转染到人羊膜细胞中激活至未分化状态的方法。两种方法均能观察到更多的高分化心肌细胞样细胞。这些细胞显示了一些作为起搏器必不可少的结构，与自动性相关的离子通道，以及形成间隙连接使动作电位传播的连接蛋白。我们已经展示了利用人类羊膜细胞建立起搏器细胞的可能性。

In order to perform pacemaker cell replacement therapy using amnion delivered cells as a new therapy instead of the mechanical pacemaker implantation, we developed some methods that to purify Nanog positive cells from Human amnion delivered cells, and gene transfection of Oct4 into Human amnion delivered cells to activate into undifferentiated state. In both methods, more high rate and highly differentiated cardiomyocyte-like cells were observed. These cells showed some structures that are essential to perform as a pacemaker, ion channels that associate with automaticity, and Connexin which forms Gap junction making action potential propagate. We have showed the possibility of the pacemaker cells establishment using Human amniotic delivered cells.