Identification of the novel molecular marker for oligodendroglial tumor using induced differentiation system against glioma stem cell.

恶性胶质瘤预后差，胶质瘤干细胞可能在其中起关键作用。我们用球体法从手术标本中建立了胶质瘤干细胞系。利用这些胶质瘤干细胞，我们生物学分析了两个新的分子，N-myc下游相关基因（NDRG）和EVI 1。(1)胶质瘤干细胞不表达NDRG。然而，在血清诱导分化后，胶质瘤细胞强烈表达NDRG。(2)EVI 1是一种转录因子。基因敲除小鼠表现出中枢神经系统的异常。我们已经证明，在胶质瘤干细胞中敲低EVI 1，并发现Notch信号转导系统被强烈抑制。这些新发现对于建立针对恶性胶质瘤的新治疗方法可能很重要。

Malignant gliomas show dismal prognosis and glioma stem cell may play a pivotal role on this issue. We established glioma stem cell lines from surgical samples using spheroid method. Using these glioma stem cells, we biologically analyzed 2 novel molecules, N-myc downstream-related gene (NDRG) and EVI1. (1) Glioma stem cell did not express NDRG. However, after differentiation in response to serum, glioma cell strongly express NDRG. (2) EVI1 is a transcriptional factor. Knock-out mice show abnormality in central nervous system. We have shown that knock-down of EVI1 in glioma stem cell and found that Notch signal transduction system was strongly supressed. These novel findings might be important for establishing new treatment against malignant glioma.