Development of a novel animal model of endometriosis using magnetic materials

本研究项目的目的是使用磁性材料开发一种新的子宫内膜异位症动物模型，该模型能够通过体内成像对子宫内膜异位症病变进行非侵入性和实时评估。我们首先开发了一种慢病毒载体，能够在感染细胞中表达非功能性表面标记物和荧光和发光蛋白。然后，我们成功地分离出感染的子宫内膜细胞，并使用磁性材料将它们聚集到体内的特定部位。最后，我们能够使用体内成像系统以非侵入性和实时的方式评估重建病变的行为。

The aim of this research project was to develop a novel animal model of endometriosis using magnetic materials which enables non-invasive and real-time of assessment of the endometriotic lesion by in vivo imaging. We first have developed a lentivirus vector enabling the expression of a non-functional surface marker and fluorescent and luminescent proteins in infected cells. We then successfully isolated the infected endometrial cells and accumulated them to the specific site in vivo using magnetic materials. Finally, we were able to assess the behavior of the reconstructed lesion in non-invasive and real-time manner using in vivo imaging system.