Biomakers associated with ventilator associated lung injury

与呼吸机相关肺损伤相关的生物制造商

• 批准号: 20390460
• 负责人: HASHIMOTO Satoru
• 金额: $ 9.32万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390460/
• 关键词: 人工呼吸関連肺損傷; ラミニン5γ2断片; 細胞外ATP; neutral endopeptidase(NEP)活性; モエシン; 急性肺損傷; neutral endopeptidase; Laminin5; ATP、細胞外; 肺胞上皮被覆液; ATP。細胞外

We studied several biomarkers that could predict the mortality or efficacy of therapy in patients with acute lung injury or acute respiratory distress syndrome (ALI/ARDS). For this aim, we conducted clinical and basic studies. In clinical studies, we sampled blood and alveolar epithelial lining fluid which was facilitated by the method of bronchoalveolar microsampling in ALI/ARDS patients. As a result, we found significantly higher amount of amino-terminal fragment of laminin gamma2-chain in blood and in epithelial lining fluids of patients with ALI/ARDS. And also, neutral endopeptidase (NEP) activity in ALI/ARDS patients were significantly lower compared to control group. In basic studies we conducted the experiments in similar lung injurious settings. In addition, extracellular ATP and moesin expression in association with lung injury were studied using mouse model. These studies confirmed our hypothesis that these biomarkers increase or decrease in accordance with the severity of the disease. Thus these biomarkers should be examined in a larger clinical trial whether it could useful for the physicians.

我们研究了几种可以预测急性肺损伤或急性呼吸窘迫综合征（ALI/ARDS）患者的死亡率或治疗效果的生物标志物。为此，我们进行了临床和基础研究。在临床研究中，我们通过支气管肺泡微量采样方法对 ALI/ARDS 患者采集了血液和肺泡上皮内壁液体样本。结果，我们发现 ALI/ARDS 患者的血液和上皮衬里液中层粘连蛋白 γ2 链氨基末端片段的含量明显较高。而且，ALI/ARDS 患者的中性肽链内切酶 (NEP) 活性显着低于对照组。在基础研究中，我们在类似的肺部损伤环境中进行了实验。此外，还使用小鼠模型研究了与肺损伤相关的细胞外 ATP 和 moesin 表达。这些研究证实了我们的假设，即这些生物标志物根据疾病的严重程度而增加或减少。因此，应该在更大规模的临床试验中检查这些生物标志物是否对医生有用。

项目成果

Laminin γ2 fragments are increased in the circulation of patients with early-phase acute lung injury.

早期急性肺损伤患者循环中层粘连蛋白γ2片段增加。

• 发表时间: 2010
• 期刊: Intensive Care Med
• 作者: Katayama M;Ishizaka A;Kotani K;Ware L;Matthay M;Hashimoto S.
• 通讯作者: Hashimoto S.

Acute lung inflammation and ventilator-induced lung injury caused by ATP via the P2Y receptors: an experimental study

• DOI: 10.1186/1465-9921-9-79
• 发表时间: 2008-12-13
• 期刊: RESPIRATORY RESEARCH
• 影响因子: 5.8
• 作者: Matsuyama, Hiroki;Amaya, Fumimasa;Hashimoto, Satoru
• 通讯作者: Hashimoto, Satoru

Neutrophil elastase activity in acute respiratory distress syndrome.

急性呼吸窘迫综合征中的中性粒细胞弹性蛋白酶活性。

• 发表时间: 2008
• 期刊: Respirology 13(4)
• 作者: Hashimoto S;Okayama Y;Shime N;Kimura A;Funakoshi Y;Kawabata K;Ishizaka A;Amaya F
• 通讯作者: Amaya F

診療ガイドラインセッション : ALI/ARDS診療のガイドライン

临床实践指南会议：ALI/ARDS 临床实践指南

• 发表时间: 2008
• 作者: Hashimoto S;Okayama Y;Shime N;Kimura A;Funakoshi Y;Kawabata K;lshizaka A;Amaya F;橋本悟
• 通讯作者: 橋本悟

NEUTROPHIL ELASTASE INHIBITOR IMPROVES SURVIVAL OF RATS WITH CLINICALLY RELEVANT SEPSIS

• DOI: 10.1097/shk.0b013e3181cc064b
• 发表时间: 2010-05-01
• 期刊: SHOCK
• 影响因子: 3.1
• 作者: Suda, Koichi;Takeuchi, Hiroya;Kitagawa, Yuko
• 通讯作者: Kitagawa, Yuko