Functional analysis of microRNA in oral carcinoma.

口腔癌中microRNA的功能分析。

• 批准号: 20390507
• 负责人: SHIIBA Masashi
• 金额: $ 12.31万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390507/
• 关键词: microRNA; 口腔癌; マイクロアレイ解析; 癌関連遺伝子; micro RNA; 癌関連遣伝子

The purpose of this study was to identify crucial microRNAs (miRNAs) in oral squamous cell carcinoma (OSCC), and to reveal their biological function and clinical significance. To clarify whether miRNAs are involved in OSCC, global screening using miRNA microarray analysis was performed with OSCC-derived cell lines. The altered expression profiling was validated by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. MicroRNA microarray and real-time qRT-PCR analyses identified significantly up-regulated expression of miR-196a, and down-regulated expression of miR-125b, miR-222, let-7b and let-7c in OSCC-derived cell lines. Decreased expression of miR-125b was validated in 43 of 50 OSCC samples using real-time qRT-PCR and was revealed the association with cancer cell proliferation. This relationship was confirmed by sequential experiment using transfectants, in which expression of miR-125b was elevated.

本研究旨在鉴定口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的关键microRNAs（microRNAs，miRNAs），并探讨其生物学功能和临床意义。为了阐明miRNA是否参与OSCC，使用miRNA微阵列分析对OSCC衍生的细胞系进行了全面筛选。通过实时定量逆转录酶-聚合酶链反应（qRT-PCR）分析验证改变的表达谱。microRNA微阵列和实时qRT-PCR分析鉴定了OSCC衍生细胞系中miR-196 a的显著上调表达，以及miR-125 b、miR-222、let-7 b和let-7 c的下调表达。使用实时定量PCR在50个OSCC样本中的43个中验证了miR-125 b的表达降低，并揭示了与癌细胞增殖的相关性。通过使用转染子的连续实验证实了这种关系，其中miR-125 b的表达升高。