Research of the scaffold protein JSAP1 during the differentiation of cerebellar granule cell precursors

小脑颗粒细胞前体细胞分化过程中支架蛋白JSAP1的研究

• 批准号: 20500282
• 负责人: YOSHIOKA Katsuji
• 金额: $ 2.83万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500282/
• 关键词: マウス; 小脳; 顆粒前駆細胞; シグナル伝達; 足場タンパク質; 発生・分化; MAPキナーゼ; JNK; 顆粒細胞

We performed immunohistochemical analysis and found that JSAP1, a scaffold protein for JNK signaling pathways, is expressed predominantly in the post-mitotic granule cell precursors (GCPs) of the inner external granular layer. We also showed that when stimulated by FGF receptor, JSAP1 translocates to the plasma membrane, where it recruits JNK and facilitates the activation of JNK, leading to the differentiation of cerebellar GCPs. Furthermore, we found that JSAP1-JNK signaling promotes the cell cycle exit and differentiation of cerebellar GCPs.

我们进行了免疫组织化学分析，发现JNK信号通路的支架蛋白JSAP1主要在内外部颗粒层的有丝分裂后颗粒细胞前体（GCPs）中表达。我们还发现，当受到FGF受体的刺激时，JSAP1易位到质膜，在那里招募JNK并促进JNK的激活，导致小脑gcp的分化。此外，我们发现JSAP1-JNK信号通路促进小脑GCPs的细胞周期退出和分化。

项目成果

Ablation of the scaffold protein JLP causes reduced fertility in male mice

• DOI: 10.1007/s11248-008-9191-6
• 发表时间: 2008-12-01
• 期刊: TRANSGENIC RESEARCH
• 影响因子: 3
• 作者: Iwanaga, Asuka;Wang, Guangmin;Yoshioka, Katsuji
• 通讯作者: Yoshioka, Katsuji

(Subcellular localization of the scaffold protein JSAP1 during the differentiation of cerebellar granule cell precursors)小脳穎粒前駆細胞の分化過程における足場タンパク質JSAP1の細胞内局在

（小脑颗粒细胞前体分化过程中支架蛋白JSAP1的亚细胞定位）

• 发表时间: 2010
• 作者: 佐藤時春;Enkhbat,A.;善岡克次
• 通讯作者: 善岡克次

Stress-activated mitogen kinase c-Jun NH_2 terminal kinase and p38 target Cdc25B for degradation.

应激激活的丝裂原激酶 c-Jun NH_2 末端激酶和 p38 靶向 Cdc25B 进行降解。

• 发表时间: 2009
• 期刊: Cancer Res. 69
• 作者: Uchida;S.;Yoshioka;K.;Kizu;R.;Nakagama;H.;Matsunaga;T.;Ishizaka;Y.;Poon;R.Y.;Yamashita;K.
• 通讯作者: K.

Positive feedback regulation of the scaffold protein Jsap1 gene expression during the differentiation of cerebellar granule cell precursors

小脑颗粒细胞前体细胞分化过程中支架蛋白Jsap1基因表达的正反馈调节

• 发表时间: 2009
• 作者: 佐藤時春;Enkhbat,A.;善岡克次;Kunikazu KOBAYASHI;兼平龍;佐藤時春
• 通讯作者: 佐藤時春