An examination of biological rhythms based on hormone secretion in saliva as parameters of morning motivation or fatigue

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Adolescent in Japan suffering from psychosomatic complaints often have chronotypic problems. In the current study, 15 healthy students between 21 and 22 years of age were examined for physiological parameters of chronotypes based on melatonin and growth hormone secretion patterns, using a fluorescence enzyme immunoassay. Salivary samples were collected from subjects at home five times each day. In addition, the subjects rated their psychosomatic symptoms twice a day. The irregular circadian rhythm of melatonin (ICR) group showed more psychosomatic complaints as compared with the regular circadian rhythm (RCR) group, especially for anxiety. Psychosomatic symptoms, especially anxiety, might be associated with melatonin and growth hormone rhythms, which can be altered by basic lifestyle habits even in healthy students.